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MAB2029

Sigma-Aldrich

硫酸软骨素蛋白聚糖抗体,克隆9.2.27

clone 9.2.27, Chemicon®, from mouse

别名:

Anti-CSPG4A, Anti-MCSP, Anti-MCSPG, Anti-MEL-CSPG, Anti-MSK16, Anti-NG2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

9.2.27, monoclonal

物種活性

human

製造商/商標名

Chemicon®

技術

flow cytometry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable

同型

IgG2a

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... CSPG4(1464)

特異性

看识别250 kDa的成熟硫酸软骨素蛋白聚糖核心糖蛋白以及210、220和240 kDa的前体多肽(Bumoi et al., 1984)。可与人黑色素瘤、神经胶质瘤和增殖的脑内皮细胞反应。

免疫原

耗尽纤连蛋白并由小扁豆凝集素-Sepharose结合的人M14黑色素瘤细胞提取物(Morgan et al., 1981)。

應用

可使用这种硫酸软骨素蛋白聚糖抗体(克隆9.2.27)检测硫酸软骨素蛋白聚糖,该抗体经验证可用于FC、IF & IP。
培养细胞的免疫荧光显微镜检查(Bumoi et al., 1984; Schrappe et al., 1992)。此外,Legg J et al (2003) Development 130:6049-6063 {3% PFA3固定的冷冻切片}。

免疫电子显微镜检查(Bumoi et al., 1984)

免疫沉淀:1μg/10E6个细胞(Bumoi et al., 1984; Morgan et al., 1981)

FACS分析:1-2μg/10E6个细胞

黑色素瘤细胞扩散的功能性阻滞(Morgan et al., 1981),裸鼠体内的神经胶质瘤细胞增殖3和肿瘤生长(Morgan et al., 1981; Schrappe et al., 1992)。

最佳工作稀释度必须由最终用户进行确定。
研究子类别
ECM 蛋白
研究类别
细胞结构

外觀

形式:纯化
纯化的免疫球蛋白,溶于含0.25M NaCl和0.1%叠氮化钠的0.02M PBS(pH 7.6)中。

儲存和穩定性

未稀释的等分试样在2-8°C可保存6个月。

其他說明

浓度:请参考批次特异性浓缩物的检验报告。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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