推荐产品
生物源
mouse
品質等級
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
9.2.27, monoclonal
物種活性
human
製造商/商標名
Chemicon®
技術
flow cytometry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
同型
IgG2a
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... CSPG4(1464)
特異性
看识别250 kDa的成熟硫酸软骨素蛋白聚糖核心糖蛋白以及210、220和240 kDa的前体多肽(Bumoi et al., 1984)。可与人黑色素瘤、神经胶质瘤和增殖的脑内皮细胞反应。
免疫原
耗尽纤连蛋白并由小扁豆凝集素-Sepharose结合的人M14黑色素瘤细胞提取物(Morgan et al., 1981)。
應用
可使用这种硫酸软骨素蛋白聚糖抗体(克隆9.2.27)检测硫酸软骨素蛋白聚糖,该抗体经验证可用于FC、IF & IP。
培养细胞的免疫荧光显微镜检查(Bumoi et al., 1984; Schrappe et al., 1992)。此外,Legg J et al (2003) Development 130:6049-6063 {3% PFA3固定的冷冻切片}。
免疫电子显微镜检查(Bumoi et al., 1984)
免疫沉淀:1μg/10E6个细胞(Bumoi et al., 1984; Morgan et al., 1981)
FACS分析:1-2μg/10E6个细胞
黑色素瘤细胞扩散的功能性阻滞(Morgan et al., 1981),裸鼠体内的神经胶质瘤细胞增殖3和肿瘤生长(Morgan et al., 1981; Schrappe et al., 1992)。
最佳工作稀释度必须由最终用户进行确定。
免疫电子显微镜检查(Bumoi et al., 1984)
免疫沉淀:1μg/10E6个细胞(Bumoi et al., 1984; Morgan et al., 1981)
FACS分析:1-2μg/10E6个细胞
黑色素瘤细胞扩散的功能性阻滞(Morgan et al., 1981),裸鼠体内的神经胶质瘤细胞增殖3和肿瘤生长(Morgan et al., 1981; Schrappe et al., 1992)。
最佳工作稀释度必须由最终用户进行确定。
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
外觀
形式:纯化
纯化的免疫球蛋白,溶于含0.25M NaCl和0.1%叠氮化钠的0.02M PBS(pH 7.6)中。
儲存和穩定性
未稀释的等分试样在2-8°C可保存6个月。
其他說明
浓度:请参考批次特异性浓缩物的检验报告。
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Not finding the right product?
Try our 产品选型工具.
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 2
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Aging cell, 17(3), e12728-e12728 (2018-02-18)
The population of brain pericytes, a cell type important for vessel stability and blood brain barrier function, has recently been shown altered in patients with Alzheimer's disease (AD). The underlying reason for this alteration is not fully understood, but progressive
Journal of visualized experiments : JoVE, (203) (2024-01-29)
The microSiM (µSiM) is a membrane-based culture platform for modeling the blood-brain barrier (BBB). Unlike conventional membrane-based platforms, the µSiM provides experimentalists with new capabilities, including live cell imaging, unhindered paracrine signaling between 'blood' and 'brain' chambers, and the ability to
Angiogenesis, 16(2), 309-327 (2012-11-06)
Sprouting of angiogenic perivascular cells is thought to be highly dependent upon autocrine and paracrine growth factor stimulation. Accordingly, we report that corneal angiogenesis induced by ectopic FGF implantation is strongly impaired in NG2/CSPG4 proteoglycan (PG) null mice known to
Glia, 65(11), 1809-1820 (2017-08-11)
Dysfunctional astrocytes are increasingly recognized as key players in the development and progression of mesial temporal lobe epilepsy (MTLE). One of the dramatic changes astrocytes undergo in MTLE with hippocampal sclerosis (HS) is loss of gap junction coupling. To further
Acta neuropathologica communications, 1, 7-7 (2013-11-21)
Neuron Glial 2 (NG2) cells are glial cells known to serve as oligodendrocyte progenitors as well as modulators of the neuronal network. Altered NG2 cell morphology and up-regulation as well as increased shedding of the proteoglycan NG2 expressed on the
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门