Extending the primary incubation step beyond the specified time in the kit protocol is not something that has been tested. A longer incubation time could potentially lead to decreased performance, including poor recovery or increased variability in assay characteristics. It may also result in higher nonspecific binding, leading to elevated signals in background wells and a loss of sensitivity.
HTH17MAG-14K
MILLIPLEX® Human TH17磁珠板-免疫学多重检测试剂
Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
别名:
Human Th17 cytokine multiplex kit, Luminex® human Th17 cytokine immunoassay, Millipore human Th17 cytokine panel
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About This Item
UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
质量水平
种属反应性
human
制造商/商品名称
Milliplex®
assay range
accuracy: 79-107%
standard curve range: 1-5000 pg/mL
(IL-10)
standard curve range: 10-40,000 pg/mL
(IFNγ)
standard curve range: 12-50,000 pg/mL
(IL-17A & IL-2)
standard curve range: 24-100,000 pg/mL
(IL-17 & IL-4)
standard curve range: 3-10,000 pg/mL
(IL-6 & TNFα)
standard curve range: 336-1,500,000 pg/mL
(IL-23)
standard curve range: 37-150,000 pg/mL
(IL-22 & TNFβ)
standard curve range: 488-2,000,000 pg/mL
(IL-17E/IL-25)
standard curve range: 49-200,000 pg/mL
(IL-28a & IL-31)
standard curve range: 5-20,000 pg/mL
(MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33)
standard curve range: 6-25,000 pg/mL
(IL-5)
standard curve range: 61-250,000 pg/mL
(GM-CSF & IL-27)
standard curve range: 7-30,000 pg/mL
(IL-13)
standard curve range: 9-35,000 pg/mL
(IL-9)
技术
multiplexing: suitable
检测方法
fluorometric (Luminex xMAP)
运输
wet ice
一般描述
CD4 T-辅助细胞是适应性免疫的主要参与者。基于它们的转录因子和分泌的细胞因子的表达谱，这些细胞被分为主要的亚类Th1，Th2，Th17和调节性（Treg）T细胞 。由IL-12诱导并由IFNγ增强的Th1细胞分泌激活巨噬细胞的IFNγ。在IL-4诱导下，Th2细胞主要产生IL-4，IL-5，IL-13，IL-25和IL-17E，在过敏中起作用。由TGFβ诱导的Treg细胞产生抗炎细胞因子IL-10和TGFβ以及在控制T细胞反应中起作用，从而防止自身免疫反应。Th17细胞是最近发现的以IL-17产生为特征的亚类，在人中由TGFβ诱导，与IL-23和IL-1β结合，在小鼠中由TGFβ，IL-6和IL-21结合诱导。活化的Th17细胞分泌IL-17A、IL-17F、IL-21、IL-22和TNFα促进组织炎症。
Th17细胞参与细胞外细菌和真菌的清除。它们在肠固有层中含量丰富，作为抵抗入侵病原体的屏障。过量的Th17细胞与几种自身免疫性疾病的发病机理有关，包括多发性硬化，银屑病，青少年糖尿病，类风湿关节炎，克罗恩′病和自身免疫葡萄膜炎。此外，Th17细胞在肿瘤的发展，进展和转移中起着重要作用，可能在促进和抑制肿瘤的生长中都起着重要作用。
MILLIPLEX ®人TH17珠面板是一种25重试剂盒，用于同时定量血清、血浆或培养上清液样品中的以下分析物：GM-CSF、IFN-γ、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-9、IL-10、IL-12（p70）、IL-13、IL-15、IL-17A、IL-17F、IL-17E/IL-25、IL-21、IL-22、IL-23、IL-27、IL-28A、IL-31、IL-33、MIP-3α/CCL20、TNF-α和TNFβ。
Luminex® xMAP®平台使用磁珠免疫分析格式，以实现理想的速度和灵敏度，同时定量多种分析物，从而显著提高生产力，同时节省宝贵的样品量。
面板类型：细胞因子/趋化因子
Th17细胞参与细胞外细菌和真菌的清除。它们在肠固有层中含量丰富，作为抵抗入侵病原体的屏障。过量的Th17细胞与几种自身免疫性疾病的发病机理有关，包括多发性硬化，银屑病，青少年糖尿病，类风湿关节炎，克罗恩′病和自身免疫葡萄膜炎。此外，Th17细胞在肿瘤的发展，进展和转移中起着重要作用，可能在促进和抑制肿瘤的生长中都起着重要作用。
MILLIPLEX ®人TH17珠面板是一种25重试剂盒，用于同时定量血清、血浆或培养上清液样品中的以下分析物：GM-CSF、IFN-γ、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-9、IL-10、IL-12（p70）、IL-13、IL-15、IL-17A、IL-17F、IL-17E/IL-25、IL-21、IL-22、IL-23、IL-27、IL-28A、IL-31、IL-33、MIP-3α/CCL20、TNF-α和TNFβ。
Luminex® xMAP®平台使用磁珠免疫分析格式，以实现理想的速度和灵敏度，同时定量多种分析物，从而显著提高生产力，同时节省宝贵的样品量。
面板类型：细胞因子/趋化因子
特异性
交叉反应性
抗体与该面板中任何其他分析物之间的交叉反应性都为无法检测或可忽略。
抗体与该面板中任何其他分析物之间的交叉反应性都为无法检测或可忽略。
应用
- 分析物：GM-CSF、IFN-γ、IL-1β、IL-2、IL-4、IL-5、IL-6、IL-9、IL-10、IL-12（p70）、IL-13、IL-15、IL-17A、IL-17F、IL-17E/IL-25、IL-21、IL-22、IL-23、IL-27、IL-28A、IL-31、IL-33、MIP-3α/CCL20、TNF-α、TNFβ
- 推荐的样品类型：血清，血浆和细胞培养样品
- 推荐的样品稀释度：纯
- 分析运行时间：过夜
- 研究类别：炎症 & 免疫学
特点和优势
通过在此面板中选择可用的分析物来设计多重试剂盒。
储存及稳定性
试剂盒组分建议的存储温度为2-8°C。
其他说明
灵敏度：有关单个生物标记物的灵敏度，请参阅试剂盒方案。
法律信息
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
靶器官
Respiratory Tract
储存分类代码
10 - Combustible liquids
Olivier Fogel et al.
The Journal of allergy and clinical immunology, 142(1), 258-268 (2017-09-17)
An interferon signature is involved in the pathogenesis of primary Sjögren syndrome (pSS), but whether the signature is type 1 or type 2 remains controversial. Mouse models and genetic studies suggest the involvement of TH1 and type 2 interferon pathways.
Danielle W Lowe et al.
Pediatric research, 82(1), 55-62 (2017-01-19)
Vitamin D has neuroprotective and immunomodulatory properties, and deficiency is associated with worse stroke outcomes. Little is known about effects of hypoxia-ischemia or hypothermia treatment on vitamin D status in neonates with hypoxic-ischemic encephalopathy (HIE). We hypothesized vitamin D metabolism
Ioannis Ilias et al.
Metabolites, 8(4) (2018-10-12)
Microdialysis (MD) can provide continuous information about tissue composition. To assess in critically ill patients adipose tissue metabolic patterns, the relationships between metabolic patterns and blood cytokine concentration associations of adipose tissue energy metabolism and clinical outcome we studied 203
Gunita Deksne et al.
Vector borne and zoonotic diseases (Larchmont, N.Y.), 21(1), 1-5 (2020-09-29)
The zoonotic parasite Dirofilaria repens has spread toward north in Europe, and cases of autochthonous dirofilariosis caused by D. repens have emerged in the Baltic countries Estonia, Latvia, and Lithuania. We conducted a review on the emergence of dirofilariosis in
Jarno Honkanen et al.
Frontiers in immunology, 11, 468-468 (2020-04-09)
Although gut bacterial dysbiosis is recognized as a regulator of beta-cell autoimmunity, no data is available on fungal dysbiosis in the children at the risk of type 1 diabetes (T1D). We hypothesized that the co-occurrence of fungal and bacterial dysbiosis
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Could you please provide information on the potential effects of leaving samples on the shaker overnight for longer than 18 hours, for example, 24-30 hours instead of the recommended 16-18 hours?
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