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HBNMAG-51K

Millipore

MILLIPLEX® 人骨磁珠板 - 骨代谢多重检测

The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN - Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α.

别名:

human bone metabolic biomarkers immunoassay panel, luminex human bone metabolism multiplex assay, millipore human bone metabolic biomarkers multiplex kit

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
Design And Price Your Kit

质量水平

200

种属反应性

human

制造商/商品名称

Milliplex®

assay range

accuracy: 82-97%
standard curve range: 0.24-1,000 pg/mL
(TNFα)
standard curve range: 1-3,000 pg/mL
(IL-1β)
standard curve range: 1-6,000 pg/mL
(ACTH & IL-6)
standard curve range: 146-600,000 pg/mL
(OC)
standard curve range: 24-100,000 pg/mL
(SOST)
standard curve range: 37-150,000 pg/mL
(FGF-23)
standard curve range: 49-200,000 pg/mL
(Leptin)
standard curve range: 5-20,000 pg/mL
(DKK-1)
standard curve range: 5-20,000 pg/mL
(PTH)
standard curve range: 61-250,000 pg/mL
(Insulin)
standard curve range: 7-30,000 pg/mL
(OPG)
standard curve range: 98-400,000 pg/mL
(OPN)

技术

multiplexing: suitable

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

一般描述

骨代谢是由成骨细胞，骨细胞和破骨细胞控制的正在进行的骨沉积和吸收的动态过程。而成骨细胞和骨细胞（被基质包围的成骨细胞）负责骨沉积，而破骨细胞则负责骨吸收。两者都需要维持骨骼结构以及充足的钙供应。为了维持这种代谢平衡，这些细胞依赖于涉及激素和细胞因子的复杂信号传导途径来实现适当的生长和分化速率。骨代谢紊乱可导致骨质疏松症、骨关节炎、类风湿关节炎、慢性肾脏疾病和骨转移等疾病。

MILLIPLEX®人骨磁珠试剂盒用于同时定量人血浆、血清或组织/细胞裂解物和培养上清液样品中以下分析物的任何或全部：促肾上腺皮质激素（ACTH)，Dickkopf-1（DKK1），IL-6，胰岛素，瘦素，TNFα，骨质疏松蛋白（OPG），骨钙素（OC），骨桥蛋白（OPN），硬化蛋白（SOST），IL-1β，甲状旁腺素（PTH）和FGF-23。该试剂盒采用96孔板，包含冻干标准混合物、两个内部检测质控品，可最多可测定38份样本，重复两次。

Luminex® xMAP®平台使用磁珠免疫分析格式，以实现理想的速度和灵敏度，同时定量多种分析物，从而显著提高生产力，同时节省宝贵的样本量。

面板类型：骨

特异性

交叉反应
对于该面板中的分析物，未观察到抗体之间的交叉反应性。

应用

  • 分析物：ACTH、DKK-1、FGF-23、IL-1β、IL-6、胰岛素、瘦素、骨钙素（OC）、骨桥蛋白（OPN）、骨保护素（OPG）、PTH、硬化蛋白（SOST）、TNFα
  • 推荐的样品类型：人血清，血浆和细胞/组织培养上清液或提取物
  • 建议的样品稀释度：每孔25μL d1:2稀释的血清或血浆；细胞/组织培养样品可以直接使用或在适当的对照培养基中稀释使用
  • 分析运行时间：在2-8℃下过夜（16-18小时）或在室温（20-25℃）下2小时。
  • 研究类别：代谢
  • 研究子类别：骨

特点和优势

通过在此面板中选择可用的分析物来设计多重试剂盒。

联系

替代：HBN1B-51K

其他说明

灵敏度：有关单个分析物的灵敏度，请参见试剂盒方案。
请联系技术服务部进行稀释度线性研究。

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

靶器官

Respiratory Tract

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

分析证书(COA)

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Sharon M Moe et al.
Circulation, 132(1), 27-39 (2015-06-11)
Patients with kidney disease have disordered bone and mineral metabolism, including elevated serum concentrations of fibroblast growth factor-23 (FGF23). These elevated concentrations are associated with cardiovascular and all-cause mortality. The objective was to determine the effects of the calcimimetic cinacalcet
Edward R Smith et al.
Scientific reports, 7(1), 5686-5686 (2017-07-20)
Calciprotein particles, nanoscale aggregates of insoluble mineral and binding proteins, have emerged as potential mediators of phosphate toxicity in patients with Chronic Kidney Disease. Although existing immunochemical methods for their detection have provided compelling data, these approaches are indirect, lack
Nigel Kurgan et al.
Frontiers in endocrinology, 11, 531926-531926 (2020-12-29)
In adults, excess adiposity has been associated with low-grade, chronic inflammation and compromised bone health, but less is known about these linkages in children. The purpose of this study was to compare the circulating levels of inflammatory cytokines, adipokines, osteokines
Chiara Giannasi et al.
International journal of medical sciences, 16(1), 23-32 (2019-01-22)
Bisphosphonates (BPs) represent the first-line treatment for a wide array of bone disorders. Despite their well-known action on osteoclasts, the effects they induce on osteoblasts are still unclear. In order to shed light on this aspect we evaluated the impact
Chiara Giannasi et al.
Stem cell research & therapy, 11(1), 521-521 (2020-12-05)
In the last years, several clinical trials have proved the safety and efficacy of adipose-derived stem/stromal cells (ASC) in contrasting osteoarthritis (OA). Since ASC act mainly through paracrine mechanisms, their secretome (conditioned medium, CM) represents a promising therapeutic alternative. ASC-CM
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Questions

1–3 of 3 Questions  

  1. Are the OPN antibodies used for detection and capture the same in all Human Milliplex kits?

    1 answer

    1. Yes, the same OPN antibody pair is used in all of the Human Milliplex kits (HANG2MAG-12K, HBNMAG-51K, HCCBP1MAG-58K, HKI4MAG-99K, HND3MAG-39K).

      Helpful?

  2. What is the specificity of Osteocalcin in the Human Bone Panel, HBNMAG-51K?

    1 answer

    1. The Osteoclacin antibodies were developed using synthetic whole molecule osteocalcins that were gamma carboxylated in the appropriate positions for each species. These antibodies were then affinity purified with peptides that were also gamma carboxylated. The standard material is also carboxylated. However, it is unknown whether the antibodies recognize non-carboxylated or under-carboxylated material, or only carboxylated material, as this has never been tested.

      Helpful?

  3. How can I determine if the Human Bone Milliplex kit is measuring total OC or uncarboxylated OC?

    1 answer

    1. The osteocalcin antibodies were developed using synthetic whole molecule osteocalcins that were gamma carboxylated and then affinity purified with peptides that were also gamma carboxylated. The standard material is also carboxylated. However, the antibodies have not been tested to determine if they recognize non-carboxylated, under-carboxylated, or only carboxylated material.

      For the Human Bone panel measuring Osteocalcin, the capture antibody is a monoclonal OC antibody that recognizes intact human osteocalcin and reacts with osteocalcin fragments corresponding to amino acids 15-31 and 20-43 of the native molecule. The detection antibody, on the other hand, is a monoclonal OC antibody that also recognizes intact human osteocalcin and reacts with osteocalcin fragments corresponding to amino acids 1-19, 7-19, and 15-31 of the native molecule.

      Helpful?

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