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EZGLP1T

Millipore

Multi Species GLP-1 ELISA Kit

measures total GLP-1 levels in 20 µl - 50 µl serum, plasma or cell culture supernatent

别名:

Glucagon-like peptide-1

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About This Item

UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.32
价格与库存信息目前不能提供

产品名称

多种类GLP-1总ELISA, EZGLP1T-36K, This Multi Species GLP-1 Total ELISA, EZGLP1T-36K, is used to measure & quantify Glucagon Like Peptide-1 levels in Metabolism research.

质量水平

200

种属反应性

human, mouse, rat

包装

kit of 1 × 96 wells

参数

20—50 μL sample volume (4hr assay)

assay range

linearity: 90-132%
recovery range: 90-110%
sensitivity: 1.5 pM
(MinDC)
standard curve range: 4.1-1000 pM
inter-assay cv: <12%
intra-assay cv: <5%

技术

ELISA: suitable

输入

sample type cell culture supernatant
sample type plasma (K2 EDTA)
sample type serum

NCBI登记号

NM_002054.2

UniProt登记号

P01275

应用

research use

检测方法

colorimetric (450nm/590nm)

运输

wet ice

储存温度

2-8°C

基因信息

human ... GCG(2641)

相关类别

一般描述

胰高血糖素样肽-1（GLP-1）是从肠 L 细胞分泌的 30 个氨基酸的肠激素。 GLP-1 序列在哺乳动物中高度保守，并响应食物摄取而以其活性形式（7-36）释放。在被 DPPIV 切割后，它迅速降解为非活性形式（9-36）。 GLP-1 通过调节胰腺分泌、减缓胃排空和降低食物摄取欲望来降低葡萄糖水平。因此，它是研究糖尿病和肥胖的重要生物标志物。该试剂盒可同时测量有效（7-36）和无效（9-36）形式的 GLP-1。

特异性

交叉反应
GLP-1 在几乎所有哺乳动物中都是保守的。
该测定法中使用的抗体对 GLP-1（7-36）和（9-36）具有特异性，与 GLP-2、GIP、胰高血糖素或泌酸调节蛋白没有交叉反应。

应用

用于检测/量化：胰高血糖素样肽-1
研究类别
代谢
这是在室温下进行的 3.5 小时检测，每孔需要 50 uL 血清、血浆或培养样品。

免责声明

仅供研究使用。不用于诊断操作。

警示用语：

Danger

危险分类

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Lindsey M Rings et al.
Journal of veterinary internal medicine, 33(6), 2752-2764 (2019-10-31)
The enteroinsular axis (EIA) comprises intestinal factors (incretins) that stimulate insulin release after PO ingestion of nutrients. Glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are the main incretins. The EIA has not been investigated in healthy neonatal foals but
Yasutaka Takeda et al.
Physiological reports, 8(11), e14469-e14469 (2020-05-31)
The short-form glucose-dependent insulinotropic polypeptide (GIP) (1-30) is released from islet alpha cells and promotes insulin secretion in a paracrine manner in vitro. However, it is not well elucidated how GIP (1-30) is involved in glucose metabolism in vivo, since

Questions

1–3 of 3 Questions  

  1. Are there any instances of cross-reactivity to Liraglutide when testing patient samples treated with Liraglude in EZGLP1T-39K, considering that these patient samples read higher than control samples?

    1 answer

    1. The kit has not been tested for cross-reactivity to liraglutide. Since liraglutide is similar to GLP-1 7-37 and our kit has 15% cross-reactivity to GLP-1 7-37, there is a possibility of some cross-reactivity.

      Helpful?

  2. What information is available for the antibodies used in the GLP-1 Total ELISA kit (EZGLP1T-36K)?

    1 answer

    1. The sequence and epitope are proprietary. The capture involves a polyclonal rabbit anti GLP-1 total, while the detection uses a monoclonal mouse anti GLP-1 (7-36), and the immunogen is mid molecule.

      Helpful?

  3. What is the cross reactivity to other forms of GLP-1?

    1 answer

    1. The assay detects GLP-1 (1-37, 1-36, 7-37), with a moderate affinity and exhibits 12-15% cross-reactivity.

      Helpful?

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