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APT110

Sigma-Aldrich

Apo-Direct TUNEL细胞凋亡检测试剂盒

The APO-DIRECT Kit is a single-step staining method for labeling DNA breaks to detect apoptotic cells by flow cytometry.

别名:

Apoptosis detection kit, TUNEL apoptosis assay, TUNEL assay kit

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.84

品質等級

物種活性

vertebrates

製造商/商標名

ApoDIRECT
Chemicon®

技術

flow cytometry: suitable

檢測方法

fluorometric

運輸包裝

wet ice

一般說明

CHEMICON APO-DIRECT细胞凋亡检测试剂盒用于单步染色法标记DNA断裂,通过流式细胞术分析凋亡细胞(Li et al. 1995)。 试剂盒含有细胞凋亡检测需要的所有试剂,包括阳性和阴性对照细胞,洗涤、反应和冲洗缓冲液,以及用于总DNA复染的碘化丙啶/RNase A溶液。

應用

APO-DIRECT试剂盒用于单步染色法标记DNA断裂,通过流式细胞术分析凋亡细胞。
研究类别
细胞凋亡 & 癌症

成分

APO-DIRECT试剂盒含有下列试剂。试剂瓶配有方便识别的不同颜色盖子。提供的试剂足以处理50份细胞悬液(包括对照细胞),每份约1 x 10E6个细胞,用于流式细胞分析。对照细胞依照程序固定(细胞固定程序),溶于 70% (v/v)乙醇。

阳性对照细胞,褐色盖

阴性对照细胞,自然色盖

洗涤缓冲液,蓝盖

反应缓冲液,绿盖

TdT酶,黄盖

F-dUTP,橙盖

冲洗缓冲液,红盖

PI/RNase染色缓冲液,棕色瓶

儲存和穩定性

警告与注意事项:

1.该试剂盒组分仅供研究使用,不可用于诊断程序。

2.阳性和阴性对照细胞含有70%(v/v)乙醇作为防腐剂,洗涤和反应缓冲液含有二甲胂酸盐作为缓冲液,冲洗和PI/RNase染色缓冲液含有0.05%(w/v)叠氮化钠作为防腐剂。 这些物质吞食有害;避免接触。 详见化学品安全说明书(Material Safety Data Sheet)。

3.TdT酶溶于50% (v/v)甘油溶液,因此置于-20℃下不会冷冻。 加热TdT酶液后,离心管子30秒,让液体都沉入管底。

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

象形圖

FlameHealth hazard

訊號詞

Danger

危險分類

Carc. 1B - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1

儲存類別代碼

3 - Flammable liquids

閃點(°F)

69.8 °F

閃點(°C)

21 °C


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