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生物来源
rabbit
质量水平
偶联物
biotin conjugate
抗体形式
F(ab′)2 fragment of affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
种属反应性
sheep
制造商/商品名称
Chemicon®
技术
ELISA: suitable
western blot: suitable
运输
wet ice
靶向翻译后修饰
unmodified
特异性
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on sheep IgG and with light chains common to most sheep immunoglobulins. No antibody was detected against non-immunoglobulin serum protein, but the antibody may cross-react with immunoglobulins from other species.
免疫原
Purified Sheep IgG.
应用
DILUTION:
1:50,000 - 1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000 - 1:100,000 for Western Blots using enzyme-conjugated streptavidin
1:500 - 1:5,000 for enzyme immunohistochemistry on tissue sections
1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry
Optimal working dilution must be determined by the end user.
1:50,000 - 1:1,000,000 for ELISA using enzyme-conjugated streptavidin
1:10,000 - 1:100,000 for Western Blots using enzyme-conjugated streptavidin
1:500 - 1:5,000 for enzyme immunohistochemistry on tissue sections
1:200 - 1:1,000 for flow cytometry and fluorescence immunohisto/cytochemistry
Optimal working dilution must be determined by the end user.
Rabbit anti-Sheep IgG Antibody, biotin-SP conjugate detects level of Sheep IgG & has been published & validated for use in ELISA & WB.
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
Whole Immunoglobulin Secondary Antibodies
外形
ImmunoAffinity Purified
Lyophilized. Buffer = 0.01M Phosphate buffer, 0.25 M NaCl with 15 mg/mL BSA and 0.05% sodium azide.
储存及稳定性
Maintain lyophilized product at 2-8°C for up to 12 months from date of receipt. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles. Expiration date: one year from date of reconstitution.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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