ABN78B

Anti-NeuN (rabbit) Antibody, Biotin Conjugate

from rabbit, biotin conjugate

• 别名: NEUronal Nuclei, Neuronal Nuclei
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: rabbit
• 质量水平: 100
• 偶联物: biotin conjugate
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 种属反应性: mouse
• 技术: immunohistochemistry: suitable
• 运输: wet ice
• 靶向翻译后修饰: unmodified
• 基因信息: mouse ... Rbfox3(52897)

说明

一般描述

ABN78 is a rabbit polyclonal version of the Anti-NeuN, clone A60 (MAB377), NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

特异性

This antibody recognizes NeuN at the N-terminus. The unconjugated version if this antibody (ABN78) has been shown to work in human and rat

免疫原

Epitope: N-terminus

GST-tagged recombinant protein corresponding to mouse NeuN.

应用

Anti-NeuN (rabbit) Antibody, Biotin Conjugate detects level of NeuN (rabbit) & has been published & validated for use in IH.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

质量

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected NeuN in adult mouse brain tissue.

目标描述

The unconjugated parent antibody (Cat. No. ABN78) has an observed molecular weight at ~46/48 kDa An uncharacterized band appears at ~62 kDa in some lysates.

外形

Affinity purified

Purified rabbit polyclonal conjugated to Biotin in PBS with 0.1% sodium azide and 15 mg/mL BSA.

储存及稳定性

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

分析说明

Control
Adult mouse brain tissue

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10 - Combustible liquids
• WGK: WGK 2
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable