ABN1676M

Anti-Calcipressin-1

serum, from rabbit

• 别名: RCAN-1, Down syndrome critical region protein 1 homolog, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: rabbit
• 质量水平: 100
• 抗体形式: serum
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 种属反应性: rat, human
• 包装: antibody small pack of 25 μL
• 技术: immunocytochemistry: suitable; western blot: suitable
• 同位素/亚型: IgG
• NCBI登记号: NP_714946
• UniProt登记号: Q6IN33
• 靶向翻译后修饰: unmodified
• 基因信息: rat ... Rcan1(266766)

说明

一般描述

Calcipressin-1 (Uniprot: Q6IN33; also known as Down syndrome critical region protein 1 homolog, Myocyte-enriched calcineurin-interacting protein 1, MCIP1, Regulator of calcineurin 1, RCAN-1) is encoded by the Rcan1 (also known as Dscr1) gene (Gene ID: 266766) in rat. Calcipressin-1 is a cytoplasmic protein that directly binds to the catalytic domain of calcineurin and acts as its negative regulator. When phosphorylated at the conserved FLISPP motif its capacity to inhibit calcineurin is increased. This phosphorylation also controls the half-life of calcipressin-1 by accelerating its degradation. It is a calcium-responsive enzyme, which is reported to dephosphorylate the nuclear factor of activated T cells (NFAT), which promotes its nuclear translocation and links calcium signaling to transcriptional regulation. Calcipressin-1 displays a quick turnover with a half-life of less than 3 hours. However, a Ser to Ala mutation at Ser163 prolongs its half-life to more than 9 hours. (Ref.: Genesca, L et al. (2003). Biochem. J. 374(2); 567-575).

特异性

This rabbit polyclonal antibody detects calcipressin-1 (RCAN-1) in human and rat cells. It targets an epitope with in 11 amino acids from the C-terminal half.

免疫原

A linear peptide corresponding to 11 amino acids from the C-terminal half of rat Calcipressin-1.

Epitope: unknown

应用

Anti-Calcipressin-1 Antibody, Cat. No. ABN1676, is a highly specific rabbit polyclonal antibody that targets Calcipressin-1 and has been tested in Immunocytochemistry and Western Blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected Calcipressin-1 in cultured rat sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119).

Immunocytochemistry Analysis: A representative lot detected Calcipressin-1 in sympathetic neurons (Patel, A., et. al. (2015). Nat Commun. 6:10119).

质量

Evaluated by Immunocytochemistry in SH-SY5Y cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Calcipressin-1 in SH-SY5Y cells.

目标描述

22.95 kDa calculated.

外形

Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable