ABE1312

Anti-SF3b155 (SAP 155) Antibody

from rabbit, purified by affinity chromatography

• 别名: Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, SF3b155, Spliceosome-associated protein 155, SAP 155
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, human, rat
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_036565
• UniProt登錄號: O75533
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SF3B1(23451)

说明

一般說明

Splicing factor 3B subunit 1, also known as Pre-mRNA-splicing factor SF3b 155 kDa subunit (SF3b155), Spliceosome-associated protein 155 (SAP 155), and encoded by the gene name SF3B1 or SAP155, is a member of the SF3B1 family . SF3B155 (SAP 155) functions a s a subunit of the splicing factor SF3B required for ′A′ complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3B155 (SAP 155) has been implied to be involved in the assembly of the ′E′ complex, and also may anchor U2 snRNP to the pre-mRNA. Additionally, SF3B155 (SAP 155) belongs to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. SF3B155 (SAP 155) has been identified in the spliceosome C complex, as a component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Phosphorylation of SF3B155 (SAP 155) occurs concomitantly with the splicing catalytic steps. The phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis. SF3B155 (SAP 155) is located in the nucleus speckle and during mitosis, it is transiently dispersed from the nuclear speckles to the cytoplasm.

免疫原

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to human SF3b155 (SAP 155) near the N-terminus.

應用

Anti-SF3b155 (SAP 155) is an antibody against SF3b155 (SAP 155) for use in WB, ICC.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected SF3b155 (SAP 155) in HeLa, A431, and NIH/3T3 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected SF3b155 (SAP 155) in 10 µg of HeLa cell lysate.

標靶描述

~160 kDa observed

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable