71789

Overnight Express^™ Autoinduction NMR Medium - ¹⁵N, ¹³C - Novagen

• 分類程式碼代碼: 12352200
• NACRES: NA.54

属性

• 製造商／商標名: Novagen^®
• 品質等級: 100
• 儲存條件: OK to freeze

说明

一般說明

Overnight Express™ Autoinduction NMR-¹⁵N, -¹³C, when supplemented with ¹³C-labeled glycerol (Cat. No. CLM1510EMD), provides reagents to make 1 L of defined autoinducing liquid culture medium used to produce [U-¹⁵N, U-¹³C]-labeled proteins for NMR spectroscopy. The autoinduction method is based on the differential metabolism of carbon sources to promote growth to high cell density and to automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG). The system includes four concentrated sterile solutions: OnEx Dual-NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by induction; OnEx NMR Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis; OnEx Solution 3 provides magnesium for maximal cell density; OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and satisfy the requirements of metal-containing target proteins, even at high expression levels. The kit also includes ¹⁵N-labeled solid ammonium chloride, ¹³C-labeled solid glucose, and reagents to make a non-inducing starter medium, for staged growth without expression and for stable storage of clones. Efficient ¹³C protein labeling requires ¹³C-glycerol, which must be purchased separately. Addition of the four liquid components and sterile solutions of ¹⁵N ammonium chloride, ¹³C glucose, and ¹³C glycerol to sterile water results in a chemically- defined medium promoting high cell densities, autoinduction of expression, and efficient labeling of target proteins.

警告

Toxicity: Harmful

儲存和穩定性

Ship Code: Multiple Storage Conditions
Storage: Multiple storage requirements

法律資訊

Express is a trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 象形圖: GHS08,GHS05,GHS07
• 訊號詞: Danger
• 危險聲明: H290,H302,H317,H319,H350,H412
• 防範說明: P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• 危險分類: Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Irrit. 2 - Met. Corr. 1 - Skin Sens. 1
• 儲存類別代碼: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects