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一般說明
BugBuster®预混液可用于高效的蛋白纯化及其它相关应用。它可取代高压破菌(French press)或超声波处理等机械方法。提供两种包装规格，分别包含足以从20 g或100 g细胞糊中提取蛋白的试剂。
應用
BugBuster®预混液的应用实例：
- 在蛋白纯化实验中重悬和裂解细胞沉淀
- 提取免疫印迹用蛋白
- 作为细菌裂解缓冲液用于制备包涵体
生化／生理作用
BugBuster®蛋白提取试剂专门用于温和地破坏大肠杆菌细胞壁以释放可溶性蛋白。利用混合去垢剂的独特配方有效地穿破细胞壁但不会使可溶性蛋白变性。结合BugBuster试剂、rLysozyme溶液和Benzonase核酸酶，BugBuste预混液对蛋白的提取效率更高，获得的蛋白提取物下游处理更加方便。
特點和優勢
- 为目标表达蛋白释放提供方便、快捷、高性价比的解决方案。
- 该预混液无需稀释或进行额外的分离操作。
- 革兰氏阳性和革兰氏阴性细菌的高效裂解
- 最大限度回收活性可溶性蛋白
- 能穿透细胞壁而不使可溶性蛋白变性
- 用于制备高纯度包涵体
- 与蛋白酶和磷酸酶抑制剂混合物兼容
警告
毒性：标准处理（A）
法律資訊
BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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相关内容
常用的细胞裂解和蛋白质提取方法包括表面活性剂溶解法、冻融裂解法、渗透压休克法、超声破碎法、酶解细胞裂解法以及机械破碎技术（如Dounce、Polytron、研钵和研棒均质化）。
Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.
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