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Merck

B7435

Sigma-Aldrich

CelLytic B 细胞裂解试剂

For bacterial cell lysis, standard strength

别名:

Cell lysis reagent

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About This Item

MDL號碼:
分類程式碼代碼:
41116134
NACRES:
NA.77

應用

CelLytic B 细胞裂解试剂已用于细胞裂解,用于提取辅酶 Q10、 重组 Src 激酶相关磷蛋白 1-谷胱甘肽 S -转移酶 (GST) 融合蛋白 和人 α-突触核蛋白。
两性离子洗涤剂的专利、非变性配方,用于细菌细胞裂解和重组蛋白提取。

特點和優勢

  • 比传统方法如超声法和溶菌酶法提取蛋白质效率高
  • 可放大 1-25 克细菌细胞浆
  • 不干扰下游应用,如亲和层析、IP 和免疫印迹
  • 与蛋白酶抑制剂、抑制剂混合物、离液剂、盐、螯合剂、还原剂相容

其他說明

在 40 mM Trizma ® HCl (pH 8.0) 中配制的洗涤剂混合物。

法律資訊

此产品受美国专利号7,282,475 B2管辖,仅针对研究用途出售。如需商业化使用,需另行征得许可。
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Ken Motohashi
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Seamless ligation cloning extract (SLiCE) is a simple and efficient method for DNA assembly that uses cell extracts from the Escherichia coli PPY strain, which expresses the components of the λ prophage Red/ET recombination system. This method facilitates restriction endonuclease
Ca2+ increases the specific coenzyme Q10 content in Agrobacterium tumefaciens
Ha SJ, et al.
Bioprocess and Biosystems Engineering, 32(5), 697-700 (2009)
Immune adaptor protein SKAP1 (SKAP-55) forms homodimers as mediated by the N-terminal region
Raab M, et al.
BMC Research Notes, 11(1), 869-869 (2018)
Prion-like seeding of misfolded alpha-synuclein in the brains of dementia with Lewy body patients in RT-QUIC
Sano K, et al.
Molecular Neurobiology, 55(5), 3916-3930 (2018)
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Serine 129 (S129) phosphorylation of α-synuclein (αSyn) is a central feature of Lewy body (LB) disease pathology. Although the neighboring tyrosine residues Y125, Y133, and Y136 are also phosphorylation sites, little is known regarding potential roles of phosphorylation cross-talk between

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