565764

γ-Secretase Substrate, Fluorogenic

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

• 别名: γ-Secretase Substrate, Fluorogenic, NMA-GGVVIATVK(DNP)- DR DR DR-NH₂
• 经验公式（希尔记法）: C₇₀H₁₁₆N₂₆O₁₈
• 分子量: 1609.83
• 分類程式碼代碼: 12352202
• NACRES: NA.54

属性

• 品質等級: 100
• 化驗: ≥95% (HPLC)
• 形狀: lyophilized
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic); protect from light
• 顏色: yellow
• 溶解度: DMSO: 5 mg/mL; 5% acetic acid: soluble
• 運輸包裝: ambient
• 儲存溫度: −20°C

说明

一般說明

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence.

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence. Excitation max.: ~355 nm; emission max.: ~440 nm.

生化／生理作用

Cell permeable: no

Product does not compete with ATP.

Reversible: no

包裝

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

序列

NMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂

重構

Following reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他說明

Farmery, M.R., et al. 2003. J. Biol. Chem.278, 24277.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable