5.05206

Photochromic TrpA1 Channel Activator, Optovin

• 别名: Photochromic TrpA1 Channel Activator, Optovin, (E)-5-((2,5-dimethyl-1-(pyridin-3-yl)-1H-pyrrol-3-yl)methylene)-2-thioxothiazolidin-4-one, PDSP 22594, Transient Receptor Potential A1 Ion Channel Activator I, TrpA1 Channel Activator I
• 经验公式（希尔记法）: C₁₅H₁₃N₃OS₂
• CAS号: 348575-88-2
• 分子量: 315.41
• MDL编号: MFCD02219276
• UNSPSC代码: 41106609
• NACRES: NA.77

属性

• 方案: ≥95% (HPLC)
• 质量水平: 100
• 表单: powder
• 效能: 2 μM EC₅₀
• 制造商/商品名称: Calbiochem^®
• 储存条件: OK to freeze; protect from light
• 颜色: yellow
• 溶解性: DMSO: 100 mg/mL
• 储存温度: 2-8°C
• SMILES字符串: S1C(=S)NC(=O)C1=Cc2c([n](c(c2)C)c3cnccc3)C
• InChI: 1S/C15H13N3OS2/c1-9-6-11(7-13-14(19)17-15(20)21-13)10(2)18(9)12-4-3-5-16-8-12/h3-8H,1-2H3,(H,17,19,20)
• InChI key: YISGMOZSGOGYOU-UHFFFAOYSA-N

说明

一般描述

A rhodanine derivative that acts as a reversible, photoactivated (λ_{max = 415 nm), and highly TrpA1-selective channel activator, presumably by a light-activated cysteine alkylation mechanism via the α,β-unsaturated alkene group of Optovin rhodanine moiety, and enables light-induced primary murine DRG calcium response in a TrpA1-dependent and HC-030031- (Cat. No. 648485) blockable manner, allowing light-induced activation only in hTrpA1-expressing, but not TrpV1- or TrpM8-expressing, HEK293T transfectants. Its in vivo efficacy is demonstrated in light-induced zebrafish motor activity (EC50/ECmax = 2 µM/3 µM; with >1.6 µW/min 387-405 nm excitation) and low power 405 nm laser-induced head shake/twitch response in mice (0.3 µmol Optovin/20 uL DMSO/ear applied atopically before laser illumination). Exhibits little or no inhibitory activity toward hERG or a panel of 50 human and rodent CNS receptors, channels, and transporters.}

生化/生理作用

Primary Target
TrpA1

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

其他说明

Kokel, D., et al. 2013, Nat. Chem. Biol.9, 257.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable