234109-M

Colcemid

Cell synchronization agent.

• 别名: Colcemid, Demecolcine
• 经验公式（希尔记法）: C₂₁H₂₅NO₅
• CAS号: 477-30-5
• 分子量: 371.43
• MDL编号: MFCD00075459
• UNSPSC代码: 12352200

属性

• 描述: Merck USA index - 14, 2887
• 方案: ≥98% (HPLC)
• 表单: crystalline powder
• 制造商/商品名称: Calbiochem^®
• 储存条件: OK to freeze
• 溶解性: chloroform: 10 mg/mL; ethanol: soluble
• 储存温度: 10-30°C
• SMILES字符串: N(C1CCc2c(c(c(c(c2)OC)OC)OC)C3=CC=C(C(=O)C=C31)OC)C
• InChI: 1S/C21H25NO5/c1-22-15-8-6-12-10-18(25-3)20(26-4)21(27-5)19(12)13-7-9-17(24-2)16(23)11-14(13)15/h7,9-11,15,22H,6,8H2,1-5H3
• InChI key: NNJPGOLRFBJNIW-UHFFFAOYSA-N

说明

一般描述

Cell synchronization agent. Induces apoptosis by blocking mitosis in HeLa S3 cells. Depolymerizes microtubules and limits microtubule formation. Appropriate dilutions inactivate the spindle fiber mechanism during metaphase, systematically immobilizing chromosomes at the metaphase plate.

生化/生理作用

Primary Target
Depolymerizes microtubules and limits microtubule formation.

警告

Toxicity: Toxic & Carcinogenic / Teratogenic (G)

重悬

Following reconstitution, aliquot and freeze (-20°C) under an inert gas. Stock solutions are stable for up to 6 months at -20°C.

其他说明

Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.
Kanda, R., et al. 1994. J. Radiat. Res. 35, 41.
Sherwood, S.W., et al. 1994. Exp. Cell Res. 215, 373.
Nishiyama, I., and Fuji, T. 1992. Exp. Cell Res.198, 214.
Spoerri, P.E., and Roisen, F.J. 1992. J. Neurosci. Res.31, 494.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany