233900

Coelenterazine

≥90% (TLC), solid, chemiluminescence probe of the superoxide anion, Calbiochem^®

• 别名: Coelenterazine, 2-( p-Hydroxybenzyl)-6-( p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one, 2-(p-Hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-(7H)-one
• 经验公式（希尔记法）: C₂₆H₂₁N₃O₃
• CAS号: 55779-48-1
• 分子量: 423.46
• MDL编号: MFCD00467176
• UNSPSC代码: 12352200
• NACRES: NA.77

属性

• 产品名称: Coelenterazine, A cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion.
• 质量水平: 100
• 方案: ≥90% (TLC)
• 表单: solid
• 制造商/商品名称: Calbiochem^®
• 储存条件: OK to freeze; protect from light
• 颜色: brown
• 溶解性: methanol: 1 mg/mL; ethanol: soluble
• 运输: ambient
• 储存温度: −20°C
• SMILES字符串: [n]21c(nc([c]2=O)Cc5ccc(cc5)O)c([nH]c(c1)c4ccc(cc4)O)Cc3ccccc3
• InChI: 1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
• InChI key: YHIPILPTUVMWQT-UHFFFAOYSA-N

说明

一般描述

Coelenterazine (CTZ), a small prosthetic group,[1] is a luciferin or light-producing compound present in luminous marine organisms.[2][3] The imidazopyrazinone ring in CTZ is responsible for its luminous properties.[3] This chemiluminescent dye[4] is soluble in methanol and poorly soluble in water.[1] CTZ can be oxidized with the help of bovine serum albumin (BSA) and several other luciferases.[3]

应用

Coelenterazine - CAS 55779-48-1 – Calbiochem may be used:

• to determine superoxide production[1]
• to determine free radical production[4]
• in cell suspension buffer for in vivo reconstitution of aequorin[5]

生化/生理作用

Coelenterazine (CTZ) is a cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion. It can also be used for detecting changes in intracellular Ca²⁺ in cells that have been transfected with apoaequorin cDNA. CTZ functions as a powerful antioxidant. It serves as a substrate for multidrug resistance protein 1 (MDR1) P-glycoprotein (ATP-binding cassette sub-family B member 1 (ABCB1)/Pgp)[6] and can bind with apoaequorin to form the active aequorin complex.[7] It can be mainly used to quantify the formation of superoxide in neutrophils.[1]

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全信息

• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable