Lot-specific information can be found on the certificate of analysis (COA). For example, for lot #: 3952706, the specific activity is 3.0 U/mgP, and the pack size is 100 milliunits, which is equivalent to 0.1 units. Therefore, for this specific lot, there is 0.033 mg of protein. In general, for this material, each mg is about 2 to 4 units, so 100 milliunits or 0.1 unit is equivalent to about 0.025 to 0.05 mg amount of protein.
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生物来源
human neutrophils
质量水平
描述
Merck USA index - 14, 1905
方案
≥95% (SDS-PAGE)
表单
lyophilized solid (Salt-free)
比活
≥2 units/mg protein
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
技术
inhibition assay: suitable
适用性
suitable for molecular biology
应用
life science and biopharma
运输
ambient
储存温度
−20°C
基因信息
human ... CTSG(1511)
一般描述
Research area: Cell Signaling
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.[1]
Cathepsin G is stored in its active form in azurophil granules of neutrophils or monocytes. Its mature form contains one potential glycan-binding site and three disulfide bonds.[1]
应用
生化/生理作用
This protease cleaves the triple-helix structure of type I collagen in a restricted manner and degrades nonhelical regions of type I and II collagens, fibronectin, aggrecan, and elastin. Additionally, cathepsin G activates ProMMP-3 and -8.[1] Cathepsin G serves as a chemoattractant for human phagocytes and enhances the random motility of T cells. Additionally, it initiates chemotaxis, activates ERK1/2 and p38 MAPK, and facilitates the translocation of PKCζ to the cell membrane, without inducing a detectable calcium flux.[4] Other biological functions of cathepsin G include: ECM components and plasma proteins degradation, bactericidal actions, cleavage of inflammatory mediators, conversion of angiotensin I to angiotensin II, platelet activation and induction of airway submucosal gland secretion.[5]
警告
Toxicity: Standard Handling (A)
单位定义
One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol of Suc-AAPF-pNA per min at 25°C, pH 7.5.
制备说明
Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.
Reconstitute in 150 mM NaCl, 50 mM sodium acetate buffer, pH 5.5.
重悬
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -70°C.
Reconstitute in 50 mM NaOAc and 150 mM NaCl, pH 5.5.
其他说明
Glusa, E., and Adam C. 2001. Por. J. Pharmacol.133, 422.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
Shamamian, P., et al. 2001. J. Cell Physiol.189, 197.
Groutas, W.C., et al. 1993. Biochem. Biophys. Res. Commun.197, 730.
Stone, P.J., et al. 1993. Biochem. Biophys. Res. Commun.197, 130.
Groutas, W.C., et al. 1992. Arch. Biochem. Biophys.294, 144.
Maison, C.M., et al. 1991. J. Immunol.147, 921.
Travis, J. 1988. Am. J. Med.84, 37.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Dental and oral biology, biochemistry
Yamakoshi, Y, et al.
reference module in biomedical sciences (2014)
Brice Korkmaz et al.
Biochimie, 90(2), 227-242 (2007-11-21)
Polymorphonuclear neutrophils form a primary line of defense against bacterial infections using complementary oxidative and non-oxidative pathways to destroy phagocytized pathogens. The three serine proteases elastase, proteinase 3 and cathepsin G, are major components of the neutrophil primary granules that
Isabelle Migeotte et al.
Cytokine & growth factor reviews, 17(6), 501-519 (2006-11-07)
The formyl peptide receptor (FPR) family is involved in host defence against pathogens, but also in sensing internal molecules that may constitute signals of cellular dysfunction. It includes three subtypes in human and other primates. FPR responds to formyl peptides
Jun Cai et al.
iScience, 27(2), 109005-109005 (2024-02-09)
Endogenous and bacterial proteases play important roles in wound healing and infection. Analysis of alterations in the low-molecular-weight peptidome by individual enzymes could therefore provide insight into proteolytic events occurring in wounds and may aid in the discovery of biomarkers.
Dieke van der Windt et al.
FEMS immunology and medical microbiology, 66(3), 445-448 (2012-09-05)
Although the Streptococcus pneumoniae polysaccharide capsule is an important virulence factor, ~ 15% of carriage isolates are nonencapsulated. Nonencapsulated S. pneumoniae are a cause of mucosal infections. Recent studies have shown that neutrophils kill S. pneumoniae predominately through neutrophil proteases, such as elastase
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Could you please provide the item number for R: 219373? I recently purchased Human Neutrophil Cathepsin G (219373) with a concentration of 100MIU. I'm experiencing difficulty converting it to µg/ml for the required dilution. Can you assist me with this? Thank you.
1 answer-
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