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Merck
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文件

16-201

Sigma-Aldrich

蛋白质A琼脂糖/鲑鱼精子DNA,2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

别名:

ChIP agarose beads, ChIP agaraose G beads

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.32

品質等級

形狀

liquid

製造商/商標名

Upstate®

技術

ChIP: suitable

適合性

suitable for immunoprecipitation

運輸包裝

wet ice

一般說明

重组蛋白G对IgG分子的Fc区具有非常高的亲和力。将蛋白G固定在微珠上会形成一种亲和树脂,可用于从粗血清、腹水或细胞培养基中纯化IgG组分。
重组蛋白G通过溴化氰键与琼脂糖共价结合。当收集用于染色质免疫沉淀(ChIP分析)的免疫复合物时,超声处理的鲑鱼精子DNA会阻断蛋白G琼脂糖上的非特异性DNA结合位点。

包裝

2.5 mL填充珠以50%凝胶浆形式提供,最终体积为5 mL。

品質

在染色质免疫沉淀中常规评估,用于预先清除丁酸钠处理的HeLa细胞的上清液,以及随后与乙酰化组蛋白交联的DNA的免疫沉淀

外觀

含有1 mg超声鲑鱼精子DNA、2.5 mg BSA和约5 mg重组蛋白G的2.5 mL填充珠,以50%凝胶浆形式提供,每瓶最终体积为5 mL。 混悬在含有0.05%叠氮化钠的10 mM Tris-HCl,1 mM EDTA(pH 8.0)中。 液体混悬液。

儲存和穩定性

自发货之日起在4°C下可保存1年。

法律資訊

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


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Kyster K Nanan et al.
Nucleic acids research, 45(22), 12780-12797 (2017-12-16)
Actively transcribed genes adopt a unique chromatin environment with characteristic patterns of enrichment. Within gene bodies, H3K36me3 and cytosine DNA methylation are elevated at exons of spliced genes and have been implicated in the regulation of pre-mRNA splicing. H3K36me3 is
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Self-renewal and pluripotency are hallmark properties of pluripotent stem cells, including embryonic stem cells (ESCs) and iPS cells. Previous studies revealed the ESC-specific core transcription circuitry and showed that these core factors (e.g., Oct3/4, Sox2, and Nanog) regulate not only
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Light is a crucial environmental signal that promotes photomorphogenesis, the developmental process with a series of light-dependent alterations for plants to adapt various external challenges. Chromatin modification has been proposed to be involved in such light-mediated growth, but the underlying
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Human embryo lung cellular protein interacting with severe acute respiratory syndrome-coronavirus nonstructural protein-10 (HEPIS) is a novel transcriptional repressor, the expression profile and promoter activity of which have not been well studied. In the present study, in situ hybridization of

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