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一般描述
Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the
phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily
(MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members ofthis family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue
distribution and subcellular localization, and different modes of inducibility of their expression by extracellular
stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization
is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various
tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different
isoforms have been found for this gene.
phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily
(MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members ofthis family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue
distribution and subcellular localization, and different modes of inducibility of their expression by extracellular
stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization
is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various
tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different
isoforms have been found for this gene.
N-terminal 6His-tagged, recombinant, human MKP5, amino acids 320-end, expressed in E. coli.
包装
Also available in
250μg
size as catalog #
14-779M
and in
bulk
as catalog #
14-779-K
.
250μg
size as catalog #
14-779M
and in
bulk
as catalog #
14-779-K
.
质量
Routinely evaluated by the release of phosphate from the substrate 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP)
外形
Protein in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Format: Purified using Ni2+/NTA agarose.
Format: Purified using Ni2+/NTA agarose.
储存及稳定性
6 months at -70°C
其他说明
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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