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Merck
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1.10690

Millipore

GranuCult® plus DEV Lactose peptone broth

for coliforms, suitable for microbiology

别名:

Coliform enrichment broth, Lactose Peptone Broth DEV

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About This Item

分類程式碼代碼:
41106213
NACRES:
NA.74

形狀

medium granules (dehydrated (DCM))

品質等級

包裝

pkg of 500 g

製造商/商標名

GranuCult® plus

儲存條件

protect from light (Keep dry and tightly closed)

技術

microbiological culture: suitable

顏色

violet

pH值

7.0 (37 °C, 25 g/L in H2O, after autoclaving)

溶解度

25 g/L at 100 °C, clear

體積密度

700 kg/m3

應用

food and beverages
water monitoring

microbiology

儲存溫度

15-25°C

適合性

coliforms

一般說明

GranuCult® plus DEV Lactose peptone broth is suited for the enrichment and determination of the titer of coliform bacteria in the bacteriological analysis of water according to the German standard methods for the examination of water and the previous German regulations from 1990.Casein peptone and soya peptone provide nitrogen, vitamins, minerals, and amino acids for growth, while sodium chloride ensures osmotic balance. Lactose is a carbohydrate source. The pH indicator bromocresol purple is yellow below pH 5.3 (acidic) and purple above pH 6.7 (alkaline). The fermentation of lactose and the consequent acid production changes the color of the medium to yellow.

應用

GranuCult® plus DEV Lactose peptone broth is suitable for the enrichment of coliforms in the bacteriological analysis of water.

特點和優勢

  • GranuCult® offers superior granulated culture media.
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment.
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions.
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands
  • Granulation technology allows many supplements to be included, with no need to add these separately

分析報告

Appearance (clearness): clear
Appearance (colour): violet
pH-value (25 °C): 7.0 - 7.4
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): good to very good
Growth (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): good to very good
Growth (Salmonella typhimurium ATCC 14028 (WDCM 00031)): good to very good
Growth (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): medium to very good
Growth (Enterococcus faecalis ATCC 11700): medium to very good
Colour change to yellow (Escherichia coli ATCC 25922 (WDCM 00013)): +
Colour change to yellow (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Colour change to yellow (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Colour change to yellow (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): -
Colour change to yellow (Enterococcus faecalis ATCC 11700): +/-
Gas (Escherichia coli ATCC 25922 (WDCM 00013)): +
Gas (Klebsiella pneumoniae ATCC 13883 (WDCM 00097)): +
Gas (Salmonella typhimurium ATCC 14028 (WDCM 00031)): -
Gas (Aeromonas hydrophila ATCC 7966 (WDCM 00063)): -
Gas (Enterococcus faecalis ATCC 11700): -
Incubation: 24 hrs.; 35 °C; aerobic.

其他說明

  • Do not use clumped or discolored medium.
  • The prepared broth is clear and purple.
  • The pH for double-concentrated can differ from single-concentrated DEV Lactose Peptone broth.

腳註

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

法律資訊

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2


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