Eosin Y (C.I. 45380) is a CERTISTAIN® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin Y staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange. As the Eosin Y dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release and a high staining quality, with reliable staining results is guaranteed.
应用
for microscopy Certistain®
分析说明
Dye content (gravimetrically): ≥ 85 % Identity (UV/VIS-Spectrum): passes test Absorption maximum λ max (water): 515 - 518 nm Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water): 1200 - 1400 TLC-Test: passes test Loss on drying (110 °C): ≤ 8 % Suitability for microscopy: passes test
法律信息
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany