Product 1024190250 is intended for In Vitro Diagnostic Use, and the Instructions For Use mention staining cryosections but not cultured cells attached to either a culture plate or a chamber slide. Staining cultured cells would be considered "off label" use and would require validation, especially in a clinical lab setting. If the dry dye is used to prepare Oil Red O staining solution, the process for preparing the staining solution would be the same for both cryosections and cells attached to a cell culture plate or chamber slide.
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质量水平
表单
liquid
IVD
for in vitro diagnostic use
技术
microbe id | staining: suitable
转变温度
flash point -11 °C
密度
0.838 g/cm3
应用
clinical testing
diagnostic assay manufacturing
hematology
histology
储存温度
15-25°C
一般描述
Oil red O staining solution - for the detection of neutral lipids in cryosections - for microscopy, is ready-to-use staining solution that results in a brilliant, bright red staining of lipds (triglycerides), while the nuclei are counterstained blue with Hematoxylin solution acc. to Gill II (Product number 1.05175). Oil red O staining is used for the detection of neutral fat or polyethylene granula in e. g. histological tissues of human origin, in joint pathology or in cytological sample material. The stain is carried out on cryo sections of native tissue.
Approx. 200 stains can be performed with one 250 ml bottle. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Approx. 200 stains can be performed with one 250 ml bottle. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
分析说明
Suitability for microscopy: passes test
Lipids: bright red
Nuclei: blue
Lipids: bright red
Nuclei: blue
警示用语：
Danger
危险分类
Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
靶器官
Central nervous system
补充剂危害
储存分类代码
3 - Flammable liquids
WGK
WGK 2
闪点(°F)
12.2 °F
闪点(°C)
-11 °C
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Does Oil Red O staining solution 1024190250 also work for cells in culture?
1 answer-
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Need to know whether or not the Oil red O staining solution (1024190250) works in mice liver?
1 answer-
Oil Red O is soluble in water and the solubility test is conducted to 1 mg per ml. The actual maximum solubility can be higher, up to 10 mg/ml, although this may. However, the solubility test is specific to water and may differ if used in agar.
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Is the Propanol in the Oil red O staining solution Halal en Kosher ?
1 answer-
The Oil red O staining solution is prepared with ethanol and acetone. Neither of these components are certified Kosher or Halal. The staining protocol requires 2-propanol. In the product information sheet 1.09634, 2-isopropanol, is suggested. This product is also not certified Halal or Kosher. See the link below to review this protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/374/144/102419-en.pdfThe food grade 2-propanol products do offer this certification. See the link below for an example: https://www.sigmaaldrich.com/US/en/product/aldrich/w292907
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Does this work to in mouse liver frozen slices?
1 answer-
Oil Red O lacks the specificity of an antibody. The basic theory of fat or lipid staining is that the dye is dissolved in a solvent with minimal solubility. A working solution is made just prior to use to further decrease the solubility. The dye then moves from the solution with minimal solubility to the solvent where there is better solubility. In this case the dye moves from the solution to the fat or lipid being stained. It matters not whether the tissue is from a human, mouse or other animals.
This information is true to most fat or lipid stains. But with Oil Red O the solution is prepared to a working solution and no further dilution is required.
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