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生物源
rabbit
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
human
技術
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... PEMT(10400)
一般說明
PEMT (phosphatidylethanolamine N-methyltransferase) is an an important enzyme for the synthesis of membrane phospholipids. PEMT converts phosphatidylethanolamine to phosphatidyl-choline by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Inhibition of hepatic PEMT activity has been associated with Alzheimer’s disease and schizophrenia.
特異性
Other homologies: Mouse (86% sequence homology) and Rat (73% sequence homology).
This antibody recognizes the C-terminal region of human PEMT in Jurkat cell lysates. The antibody easily detected PEMT in Jurkat and L6 lysates by Western blotting, but expression was weak or not detected in A431, HeLa, HEK293, PC12, or NIH/3T3 cell lysates using very dilute antibody (0.25 μg/mL, data not shown). Positive signals in immunocytochemistry using higher concentrations of antibody (2.5 μg/mL) detected PEMT in these cell lines, suggesting differential expression levels of this protein in various cell lines.
免疫原
Epitope: C-terminus
The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human PEMT.
應用
ELISA:
This antibody was reported by an outside laboratory to be suitable for ELISA
Immunocytochemistry:
HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER).
This antibody was reported by an outside laboratory to be suitable for ELISA
Immunocytochemistry:
HeLa, A431, and NIH/3T3 (previous page) cells incubated with anti-PEMT (C-terminus) stained positively on nuclei and endoplasmic reticulum (ER).
Research Category
Epigenetics & Nuclear Function
Neuroscience
Epigenetics & Nuclear Function
Neuroscience
Research Sub Category
Chromatin Biology
Chromatin Biology
This PEMT antibody is validated for use in WB, ICC & ELISA for the detection of the PEMT protein.
品質
Western Blot Analysis:
1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown).
1:1,000 dilution of this antibody detected PEMT on 10 µg of L6 lysate (below) and Jurkat lysate (not shown).
標靶描述
~22 kDa
外觀
Protein A chromatography
Format: Purified
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
儲存和穩定性
Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
分析報告
Control
L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry)
L6 and Jurkat lysates (Western blotting), HeLa, A431 cells (Immunocytochemistry)
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
C J Walkey et al.
Biochimica et biophysica acta, 1436(3), 405-412 (1999-02-16)
Phosphatidylethanolamine is converted to phosphatidylcholine in mammalian liver by the enzyme phosphatidylethanolamine N-methyltransferase (PEMT). A form of the enzyme (PEMT2) has been isolated from rat liver, the cDNA cloned and expressed and the murine gene has been characterized and disrupted.
D J Shields et al.
Biochimica et biophysica acta, 1532(1-2), 105-114 (2001-06-23)
Phosphatidylethanolamine N-methyltransferase (PEMT) catalyzes the conversion of phosphatidylethanolamine (PE) to phosphatidylcholine (PC) in a series of three methylation reactions. Preliminary studies of PEMT in humans led to the cloning of three cDNAs each of which has a different 5' untranslated
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