06-1074

Anti-Interleukin 21 (IL-21) Antibody

from rabbit, purified by affinity chromatography

• 别名: Za11, IL-21, IL21, Interleukin-21
• UNSPSC代码: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

属性

• 生物来源: rabbit
• 质量水平: 100
• 抗体形式: affinity isolated antibody
• 抗体产品类型: primary antibodies
• 克隆: polyclonal
• 纯化方式: affinity chromatography
• 种属反应性: mouse, human
• 种属反应性（根据同源性预测）: monkey (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)
• 技术: flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• NCBI登记号: NP_068575
• UniProt登记号: Q9HBE4
• 运输: wet ice
• 基因信息: human ... IL21(59067)

说明

一般描述

Interleukin 21 (IL-21) is a cytokine that has immunoregulatory activity on cells of the immune system, including natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. IL-21 induces cell division/proliferation in its target cells and is expressed in activated human CD4+ T cells. IL-21 may promote the transition between innate and adaptive immunity and may play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. IL-21 expression is up-regulated in Th2 and Th17 subsets of T helper cells, as well as follicular T cells. Furthermore IL-21 is expressed in NK T cells regulating the function of these cells. IL-21 is also produced by Hodgkin′s lymphoma (HL) cancer cells and may explain a great deal of the behavior of classical Hodgkin′s lymphoma including clusters of other immune cells gathered around HL cells in cultures.

特异性

Cow, canine, equine, and porcine (93% sequence homology). Rat (86% sequence homology).

This antibody recognizes the C-terminus of IL-21.

免疫原

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human IL-21.

应用

Anti-Interleukin 21 (IL-21) Antibody is an antibody against Interleukin 21 (IL-21) for use in FC, IH(P) & WB.

Flow Cytometry Analysis: 5 µg from a previous lot detected IL-21 in human PBMC′s.

Immunohistochemistry Analysis: A 1:100 dilution from a previous lot detected IL-21 in mouse spleen tissue.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

质量

Evaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected IL-21 on 10 µg of K562 cell lysate.

目标描述

A molecular weight of 18 kDa was observed; however, UniProtKB/Swiss-Prot entry Q9HBE4 (IL21_HUMAN) describes 2 isoforms produced by alternative splicing: isoform 1 at 18 kDa, isoform 2 at 17 kDa

外形

Affinity purified

Purified rabbit polyclonal in PBS with 0.06% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
K562 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 储存分类代码: 10-13 - German Storage Class 10 to 13