0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
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特異性
WT1
免疫原
6His标记融合蛋白，对应人WT1（维尔姆斯瘤）的1-181位残基。
應用
抗-WT1抗体（克隆6F-H2）是一种用于检测WT1（又称维尔姆斯瘤）的小鼠单克隆抗体，目前已通过WB、ICC & IHC应用验证。
品質
已通过免疫印迹对Jurkat和Raji细胞的RIPA裂解液进行常规评估
標靶描述
55-60kDa
外觀
形式：纯化
法律資訊
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
Toru Sakairi et al.
American journal of physiology. Renal physiology, 298(3), F557-F567 (2009-12-04)
Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and proliferate in culture conditions. To determine the phenotypic characteristics of viable urinary cells derived from human subjects, we established long-term
Embryonic Stem Cells Derived Kidney Organoids as Faithful Models to Target Programmed Nephrogenesis.
Zenglai Tan et al.
Scientific reports, 8(1), 16618-16618 (2018-11-11)
The kidney is a complex organ that is comprised of thousands of nephrons developing through reciprocal inductive interactions between metanephric mesenchyme (MM) and ureteric bud (UB). The MM undergoes mesenchymal to epithelial transition (MET) in response to the signaling from
Caihua Xu et al.
PloS one, 8(8), e68837-e68837 (2013-08-13)
The Wilms' tumor suppressor gene (WT1) has been identified as an oncogene in many malignant diseases such as leukaemia, breast cancer, mesothelioma and lung cancer. However, the role of WT1 in non-small-cell lung cancer (NSCLC) carcinogenesis remains unclear. In this
Jing Zhou et al.
Biology open, 7(5) (2018-04-19)
Mesodermal populations can be generated in vitro from mouse embryonic stem cells (mESCs) using three-dimensional (3-D) aggregates called embryoid bodies or two-dimensional (2-D) monolayer culture systems. Here, we investigated whether Brachyury-expressing mesodermal cells generated using 3-D or 2-D culture systems
Kazunori Inoue et al.
The Journal of clinical investigation, 129(3), 1295-1313 (2019-02-19)
We identified 2 genes, histone deacetylase 1 (HDAC1) and HDAC2, contributing to the pathogenesis of proteinuric kidney diseases, the leading cause of end-stage kidney disease. mRNA expression profiling from proteinuric mouse glomeruli was linked to Connectivity Map databases, identifying HDAC1
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