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生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
monoclonal
物種活性
human, mouse
技術
western blot: suitable
同型
IgG2aκ
NCBI登錄號
UniProt登錄號
目標翻譯後修改
unmodified
基因資訊
human ... EED(8726)
mouse ... Eed(13626)
一般說明
Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. EED is also known to bind H3K27me3 and can allosterically stimulate the methyltransferase activity of the PRC2 complex. (Ref.: Hojfeldt, JW. et al (2018) Nat. Struc. Mol. Biol. 25 (3); 225-232).
特異性
Not tested in other species.
This antibody recognizes mouse EED, (~30-65 kDa).
免疫原
Recombinant fusion protein of full length murine EED tagged with MBP.
應用
Anti-EED Antibody, clone AA19 is a mouse monoclonal antibody for detection of EED also known as WD protein associating with integrin cytoplasmic tails 1 ,embryonic ectoderm development & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Apoptosis & Cancer
Epigenetics & Nuclear Function
Apoptosis & Cancer
Research Sub Category
Chromatin Biology
Chromatin Biology
品質
Routinely evaluated by Western Blot on A431cel lysatel.
Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.
Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.
標靶描述
Ranges from 30-65 kDa (5-6 isoforms of EED are detected).
外觀
Protein G Purified
Purified immunoglobulin in 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析報告
Control
A431 Cell Lysate
A431 Cell Lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Polycomb group proteins in cell cycle progression and cancer.
Pasini, Diego, et al.
Cell Cycle, 3, 396-400 (2004)
The central role of EED in the orchestration of polycomb group complexes.
Cao, Q; Wang, X; Zhao, M; Yang, R; Malik, R; Qiao, Y; Poliakov, A; Yocum, AK; Li, Y; Chen et al.
Nature Communications null
Adrian P Bracken et al.
Genes & development, 20(9), 1123-1136 (2006-04-19)
The Polycomb group (PcG) proteins form chromatin-modifying complexes that are essential for embryonic development and stem cell renewal and are commonly deregulated in cancer. Here, we identify their target genes using genome-wide location analysis in human embryonic fibroblasts. We find
James P Reddington et al.
Genome biology, 14(3), R25-R25 (2013-03-28)
DNA methylation and the Polycomb repression system are epigenetic mechanisms that play important roles in maintaining transcriptional repression. Recent evidence suggests that DNA methylation can attenuate the binding of Polycomb protein components to chromatin and thus plays a role in
Diego Pasini et al.
Molecular and cellular biology, 27(10), 3769-3779 (2007-03-07)
Polycomb group (PcG) proteins form multiprotein complexes, called Polycomb repressive complexes (PRCs). PRC2 contains the PcG proteins EZH2, SUZ12, and EED and represses transcription through methylation of lysine (K) 27 of histone H3 (H3). Suz12 is essential for PRC2 activity
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