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Merck

199303

Sigma-Aldrich

刃天青 钠盐

certified by the Biological Stain Commission

别名:

7-羟基-3H-吩恶嗪-3-酮-10-氧化物 钠盐

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About This Item

经验公式(希尔记法):
C12H6NNaO4
CAS号:
分子量:
251.17
Beilstein:
3794971
EC號碼:
MDL號碼:
分類程式碼代碼:
12171500
PubChem物質ID:
NACRES:
NA.47

等級

certified by the Biological Stain Commission

形狀

powder

成份

Dye content, 75%

溶解度

1 M NH4OH: 1 mg/mL

λmax

380 nm (2nd)
598 nm

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

SMILES 字串

[Na+].[O-]c1ccc2c(OC3=CC(=O)C=CC3=[N+]2[O-])c1

InChI

1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1

InChI 密鑰

IVGPGQSSDLDOLH-UHFFFAOYSA-M

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一般說明

刃天青(Resazurin)是二羟基吩噁嗪酮的N-氧化小分子衍生物。它是一种非离子染料,含有小的平面发色团。它是一种氧化还原指示剂,反应时由蓝紫色变为粉红色再变为无色。它也可用作3.5-6.5范围的pH指示剂,颜色由橙色变为蓝紫色。它的商品化形式为刃天青钠盐,也称3H-吩恶嗪-3-酮, 7-羟基-, 10-氧化物钠盐。经还原作用形成红色荧光的试卤灵(羟基吩噁嗪酮),进一步还原形成无色的氢化试卤灵。

應用

刃天青最常用作生物氧化还原指示剂。染料的生物还原减少了其氧化形式(蓝色)的含量,同时增加了荧光中间体(红色)水平。其应用包括:
  • 测定酶活力和活细胞的增殖
  • 检测乳汁中的细菌还原作用
  • 在活力测定中用作检测活细菌的培养基成分
  • 测定绵羊精液中活动精子的数目
  • 检测乳酸脱氢酶
  • 作为定量测定L-谷氨酸盐的分析试剂
用于测量活细胞的代谢活性和增殖。染料的生物还原减少了其氧化形式(蓝色)的含量,同时增加了荧光中间体(红色)水平。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


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