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58997
SUPELCOSIL™ LC-SCX (5 µm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Synonym(s):
SCX column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
$1,280.00
Please contact Customer Service for Availability
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Product Name
SUPELCOSIL™ LC-SCX HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Agency
suitable for USP L52
Quality Level
manufacturer/tradename
SUPELCOSIL™
parameter
≤70 °C temp. limit
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
25 cm × 4.6 mm
surface area
170 m2/g
matrix
silica gel, spherical particle platform
matrix active group
sulfonic acid, propyl- phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
cation exchange
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General description
The LC-SCX cation-exchange columns have strongly acidic propylsulfonic acid groups and are used for separating cations. Adjust pH, ionic strength, and organic modifier concentration to control retention and selectivity.
Application
Supelcosil™ LC-SCX HPLC column was used during the purification and characterization of 8-hydroxyguanine (oh8Gua), from Human Urine.[1] It was used as a cation exchange column, during measurement and extraction of water-soluble arsenic, freeze-dried marine animal tissues, using high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS).[2] It was also used in the chromatographic separation of arsenic compounds.[3]
Supelcosil™ LC-SCX HPLC column was used during the purification and characterization of 8-hydroxyguanine (oh8Gua), from Human Urine.[1] It was used as a cation exchange column, during measurement and extraction of water-soluble arsenic, freeze-dried marine animal tissues, using high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS).[2] It was also used in the chromatographic separation of arsenic compounds.[3] It may be used as a cation-exchange column for the separation, identification and determination of arsenic compounds in oyster tissue[4] and marine brown alga like Hijiki fuziforme using high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS).[5]
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
guard cartridge
Product No.
Description
Pricing
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Measurement of water-soluble arsenic species in freeze-dried marine animal tissues by microwave-assisted extraction and HPLC-ICP-MS.
Kirby J and Maher W.
Journal of Analytical Atomic Spectrometry, 17 (8), 838-843 (2002)
Comparison of three methods for the extraction of arsenic compounds from the NRCC standard reference material DORM-2 and the brown alga Hijiki fuziforme
Kuehnelt D, et al.
Applied Organometallic Chemistry, 15(6), 445-456 (2001)
Urinary 8-hydroxy-2'-deoxyguanosine as a biological marker of in vivo oxidative DNA damage.
Shigenaga MK
Proceedings of the National Academy of Sciences of the USA, 86 (24), 9697-9701 (1989)
Determination of arsenic compounds in earthworms.
GEISZINGER A,.
Environmental Science & Technology, 32 (15), 2238-2243 (1998)
Determination of total cationic and total anionic arsenic species in oyster tissue using microwave-assisted extraction followed by HPLC?ICP-MS
Chatterjee A
Talanta, 51(2), 303-314 (2000)
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What is the Department of Transportation shipping information for this product?
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Is there a guard column for the Supelcosil LC-SCX HPLC column, Product 58997?
1 answer-
Supelguard LC-SCX can be purchased as a guard column kit, Product No. 59509, that includes a guard cartridge in a cartridge holder, with tubing and fittings for installation in front of your analytical column. Replacement LC-SCX cartridges can be purchased as a 2 pack, Product No. 59519.
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What mobile phases can be used on the Supelcosil LC-SCX HPLC column, Product 58997?
1 answer-
Polar and non-polar HPLC solvents can be used as mobile phases for the Supelcosil LC-SCX, within pH range of 2-7. The mobile phase used for testing and shipping of the column is 0.05M KH2PO4 buffer (pH 3.5):acetonitrile (65:35). Cleaning solvents include deionized water, methanol, acetonitrile, and methylene chloride.
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What is the pressure limit for the Supelcosil LC-SCX HPLC column, Product 58997?
1 answer-
The Supelcosil LC-SCX HPLC column is packed with an aliphatic sulfonic acid functionalized silica, and can handle pressures up to 4000 psi. Most columns test out at less than 1500 psi.
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What is the buffer concentration and pH used for the mobile phase when testing Product 58997, SUPELCOSIL™ LC-SCX HPLC Column?
1 answer-
For Product 58997, SUPELCOSIL™ LC-SCX HPLC Column, the concentration of the buffer is 0.05M potassium phosphate with a pH of 3.5.
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