Skip to Content
Merck
All Photos(1)

Key Documents

58298

Supelco

SUPELCOSIL™ LC-18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Sign Into View Organizational & Contract Pricing

Select a Size

1 EA
$1,270.00

$1,270.00

Please contact Customer Service for Availability
All Photos(1)

Select a Size

Change View
1 EA
$1,270.00

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
L × I.D.:
25 cm × 4.6 mm
surface area:
170 m2/g
particle size:
5 μm
matrix active group:
C18 (octadecyl) phase
pore size:
120 Å
matrix:
silica gel, spherical particle platform

$1,270.00

Please contact Customer Service for Availability

Product Name

SUPELCOSIL™ LC-18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L1

Quality Level

100

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

extent of labeling

11.0% carbon loading

parameter

≤70 °C temp. limit
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

pH

2-7.5

application(s)

food and beverages

separation technique

reversed phase

Looking for similar products? Visit Product Comparison Guide

General description

A general purpose hydrophobic alkyl phase that is very retentive and gives good peak shape for a wide variety of compounds.

Application

SUPELCOSIL™ LC-18 HPLC Column may be used for the separation of:
  • Anthocyanins, flavonols, and carbohydrates in Tropea red onions (Allium cepa L.) during their determination by high performance liquid chromatography (HPLC) as well as HPLC coupled to mass spectrometry (MS) equipped with electrospray ionization (ESI) source.[1]
  • Sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice samples during their determination by semipreparative reversed phase HPLC.[2]
  • Aflatoxin M1 in milk and cheese samples during its determination by HPLC with post-column derivatization and fluorescence detection.[3]

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Application

Product No.
Description
Pricing

guard cartridge

Product No.
Description
Pricing

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
293517-06
293517-05
293517-04
293517-03
293517-02

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Vagish Dwibedi et al.
Biotechnology letters, 43(3), 627-644 (2020-11-08)
The present investigation primarily focusses on enhancement of resveratrol production by endophytic production from the endophytic fungus, Arcopilus aureus via one variable at a time approach (OVAT) followed by statistical approach using response surface methodology (RSM). The paper also highlights
Flavonoid and carbohydrate contents in Tropea red onions: effects of homelike peeling and storage.
Gennaro L, et al.
Journal of Agricultural and Food Chemistry, 50(7), 1904-1910 (2002)
Characterization of sinapyl derivatives in pineapple (Ananas comosus [L.] Merill) juice.
Wen L, et al.
Journal of Agricultural and Food Chemistry, 47(3), 850-853 (1999)
High-performance liquid chromatography with post-column derivatisation and fluorescence detection for sensitive determination of aflatoxin M1 in milk and cheese.
Manetta AC, et al.
Journal of Chromatography A, 1083(1-2), 219-222 (2005)

Protocols

HPLC Analysis of Vitamins, Fat Soluble (A and E), on SUPELCOSIL™ LC-18

Separation of δ-Tocopherol, analytical standard; Retinyl acetate; (+)-γ-Tocopherol; α-Tocopherol, ≥95.5%; Retinol; Vitamin A (acetate), meets USP testing specifications.

EPA Method TO-11/IP-6A and ASTM® D5197: HPLC Analysis of Aldehydes and Ketones on SUPELCOSI™ LC-18 after Collection/Desorption using LpDNPH

-Tolualdehyde; Valeraldehyde; Isovaleraldehyde

Related Content

HPLC Analysis of Fatty Acid Methyl Esters (FAMES) on SUPELCOSIL LC-18

Separation of Methyl decanoate; Methyl dodecanoate; Methyl myristate; Methyl palmitate; Methyl caprylate; Methyl oleate; Methyl linoleate; Methyl linolenate; Methyl stearate

Chromatograms

application for HPLCapplication for air monitoring, application for HPLC

Questions

1–3 of 3 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is this column comparable to the Phenomenex Luna C18 4.6 X 250mm, 5 micron

    1 answer

    1. Yes. This column is comparable to the Phenomenex Luna C18 4.6 X 250mm, 5 micron, with respect to dimensions and phase type (USP L1).

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service