MAK536

Cytotoxicity Assay Kit

sufficient for 100 assays (bioluminescent)

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): pharmaceutical
• detection method: bioluminescent
• relevant disease(s): cancer
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Adenosine 5′-triphosphate (ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency" of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery.

Features and Benefits

• As low as 50 cells can be quantified.
• Compatible with high-throughput handling systems.
• No wash and reagent transfer steps are involved.

Suitability

The kit is suitable for:

• Cell proliferation: effects of cytokines, growth factor, nutrients.
• Cytotoxicity and apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc.
• Drug discovery: high-throughput screening for anticancer drugs.

Principle

The Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability, and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317,H412
• Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3