MAK479
NADP/NADPH Assay Kit
sufficient for 100 colorimetric tests
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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General description
Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.
Features and Benefits
- The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH
- Compatible with high-throughput handling systems.
- Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.
Suitability
The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.
Principle
The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Certificates of Analysis (COA)
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