70746
Benzonase® Nuclease
Purity > 99%
Synonym(s):
Endonuclease from Serratia marcescens
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
Recommended Products
product name
Benzonase® Nuclease, Purity > 90%,
biological source
Serratia marcescens
Quality Level
recombinant
expressed in E. coli
Assay
>90% (SDS-PAGE)
form
buffered aqueous glycerol solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
concentration
25-29 units/μL
application(s)
research use
shipped in
wet ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
Benzonase® Nuclease is a geneticallyengineered endonuclease from Serratiamarcescens. It degrades all forms of DNA andRNA (single stranded, double stranded, linearand circular) while having no proteolyticactivity. It is effective over a wide range ofconditions and possesses an exceptionallyhigh specific activity. The enzyme completelydigests nucleic acids to 5′-monophosphateterminated oligonucleotides 2 to 5 bases inlength (below the hybridization limit), whichis ideal for removal of nucleic acids fromrecombinant proteins, enabling compliancewith FDA guidelines for nucleic acidcontamination. The ability of Benzonase torapidly hydrolyze nucleic acids makes theenzyme an excellent choice for viscosityreduction to reduce processing time andincrease yields of protein. For example, theenzyme is compatible with BugBuster andPopCulture Protein Extraction Reagentsand can therefore be added along with thesereagents to eliminate viscosity and removenucleic acids from E. coli extracts.
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >90% by SDS-PAGE
The enzyme consists of two subunits of30 kDa each. It is functional betweenpH 6 and 10 and from 0-42°C and requires1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, PMSF(1 mM), EDTA (1 mM) and urea (relativeactivity depends on specific conditions).Activity is inhibited by >150 mM monovalentcations, >100 mM phosphate, >100 mMammonium sulfate, or >100 mM guanidine HCl.
Benzonase Nuclease is available inultrapure (>99% by SDS-PAGE) and pure(>90%) grades at a standard concentrationof 25-29 U/µl and at a high concentration (HC)of 250 U/µl. Both preparations are free ofdetectable protease and have specific activity> 1 × 106 U/mg protein. The >99% puritygrade is tested for endotoxins and contains<0.25 EU/1000 units. The product is suppliedin 50% glycerol.Store at -20°C.
Total endotoxin:<0.25 EU/1,000 units.Purity: >90% by SDS-PAGE
Application
Used for the removal of nucleic acid from protein samples.
Biochem/physiol Actions
Digests native or heat-denatured DNA and RNA.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that causes a ΔA₂₆₀ of 1.0 in 30 minutes, which corresponds to complete digestion of 37 µg DNA.
Legal Information
Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Birgitte P S Jacky et al.
PLoS pathogens, 9(5), e1003369-e1003369 (2013-05-23)
Botulinum neurotoxin serotype A (BoNT/A) causes transient muscle paralysis by entering motor nerve terminals (MNTs) where it cleaves the SNARE protein Synaptosomal-associated protein 25 (SNAP25206) to yield SNAP25197. Cleavage of SNAP25 results in blockage of synaptic vesicle fusion and inhibition
Annika Unger et al.
Frontiers in immunology, 9, 1425-1425 (2018-07-13)
Tumors frequently exploit homeostatic mechanisms that suppress expression of IL-12, a central mediator of inflammatory and anti-tumor responses. The p40 subunit of the IL-12 heterodimer, encoded by IL12B, is limiting for these functions. Ovarian carcinoma patients frequently produce ascites which
Benjamin Strobel et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 23(10), 1582-1591 (2015-07-04)
Cytotoxicity of transgenes carried by adeno-associated virus (AAV) vectors might be desired, for instance, in oncolytic virotherapy or occur unexpectedly in exploratory research when studying sparsely characterized genes. To date, most AAV-based studies use constitutively active promoters (e.g., the CMV
Chi Zhu et al.
Molecular cell, 75(4), 791-806 (2019-07-16)
YAP/TEAD are nuclear effectors of the Hippo pathway, regulating organ size and tumorigenesis largely through promoter-associated function. However, their function as enhancer regulators remains poorly understood. Through an in vivo proximity-dependent labeling (BioID) technique, we identified YAP1 and TEAD4 protein as
Mark A Kanow et al.
eLife, 6 (2017-09-14)
Here we report multiple lines of evidence for a comprehensive model of energy metabolism in the vertebrate eye. Metabolic flux, locations of key enzymes, and our finding that glucose enters mouse and zebrafish retinas mostly through photoreceptors support a conceptually
Articles
This page lists nine frequently asked questions and answers about Benzonase® Nuclease.
The combination of BugBuster® and Lysonase™ reagents greatly enhances the release of soluble proteins from Gram-positive bacteria.
Benzonase®endonuclease efficiently removes nucleic acid contaminants from viral production, crucial for cell and gene therapies and vaccines.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service