T4330
Turbonuclease from Serratia marcescens
recombinant, expressed in E. coli
Synonym(s):
Endonuclease from Serratia marcescens
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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biological source
Serratia marcescens
Quality Level
recombinant
expressed in E. coli
form
liquid
concentration
≥200,000 units/mL
technique(s)
DNA purification: suitable
suitability
suitable for cell lysis
application(s)
life science and biopharma
storage temp.
−20°C
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General description
Endonuclease from Serratia marcescens is a dimer containing two identical monomeric units with distinct protein folds. The core contains a six-stranded antiparallel β-sheet flanked by α-helices on either side. Each monomer bears one active site. This enzyme is a magnesium-dependent nucleases.
Application
Turbonuclease from Serratia marcescens has been used for cell lysis during proximity biotinylation assay (BioID) and affinity-purification. It has also been used as a component of lysis buffer for protein extraction from cell lines for affinity purification studies.
Turbonuclease has been used in a study to assess the TY3 gag3 spacer effect on intracellular condensation and uncoating.
Used for the removal of nucleic acid from protein samples.
Biochem/physiol Actions
Endonuclease from Serratia marcescens is effective against both single- and double-stranded DNA and RNA. It mediates the digestion of the 3′ O—P bond resulting in oligonucleotides ending with 5′ monophosphate. The activity of this enzyme is known to be less affected by the reducing and chaotropic agents. It is highly stable at room temperature. This endonuclease eliminates the undesired nucleic acids in downstream processing.
Turbonuclease provides a nuclease treatment by reducing viscosity and degrading RNA, genomic DNA, baculovirus DNA, and unencapsidated vector DNA.
Digests native or heat-denatured DNA and RNA.
Unit Definition
One unit will digest sonicated salmon sperm DNA to acid-soluble oligonucleotides equivalent to a ΔA260 of 1.0 in 30 min at pH 8.0 at 37 °C.
Physical form
Supplied as a solution in 50 mM Tris-HCl, pH 8.0 and 50 mM NaCl
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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M D Miller et al.
Journal of molecular biology, 288(5), 975-987 (1999-05-18)
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