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07-823

Sigma-Aldrich

Anti-phospho eIF4E (Ser209) Antibody

Upstate®, from rabbit

Synonym(s):

eukaryotic translation initiation factor 4E, CBP, EIF4EL1, EIF4F

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (100% homologous), mouse (100% homologous), rabbit (100% homologous)

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_001968.2

UniProt accession no.

P06730

shipped in

dry ice

target post-translational modification

phosphorylation (pSer209)

Gene Information

human ... EIF4E(1977)

Specificity

Recognizes eIF4E phosphorylated on Ser209.

Immunogen

KLH conjugated synthetic peptide corresponding to amino acids surrounding Ser209 of human eIF4E. Sequence conserved in mouse and rat.

Application

Anti-phospho eIF4E (Ser209) Antibody is an antibody against phospho eIF4E (Ser209) for use in WB.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

RNA Binding Protein (RBP)

Quality

Routinely evaluated by immunoblot.

Target description

25 kDa

Physical form

ImmunoAffinity Purified

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Phosphorylation of eukaryotic initiation factor 4E (eIF4E) at Ser209 is not required for protein synthesis in vitro and in vivo
McKendrick, L, et al
European Journal of Biochemistry, 268, 5375-5385 (2001)
A J Waskiewicz et al.
Molecular and cellular biology, 19(3), 1871-1880 (1999-02-18)
Eukaryotic translation initiation factor 4E (eIF4E) binds to the mRNA 5' cap and brings the mRNA into a complex with other protein synthesis initiation factors and ribosomes. The activity of mammalian eIF4E is important for the translation of capped mRNAs
B Joshi et al.
The Journal of biological chemistry, 270(24), 14597-14603 (1995-06-16)
Initiation factor 4E (eIF-4E) binds to the m7GTP-containing cap of eukaryotic mRNA and facilitates the entry of mRNA into the initiation cycle of protein synthesis. eIF-4E is a phosphoprotein, and the phosphorylated form binds to mRNA caps 3-4-fold more tightly
Phosphorylation of eukaryotic initiation factor 4E markedly reduces its affinity for capped mRNA
Scheper, Gert C, et al
The Journal of Biological Chemistry, 277, 3303-3309 (2002)
A Flynn et al.
The Journal of biological chemistry, 270(37), 21684-21688 (1995-09-15)
Ser-53 has previously been considered the major phosphorylation site in eukaryotic initiation factor (eIF)-4E, and this appeared to be supported by studies using a S53A mutant. Recently, however, several lines of evidence have indicated that Ser-53 might not be the
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