Skip to Content
Merck
All Photos(2)

Documents

MAK165

Sigma-Aldrich

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (red fluorescence)

Synonym(s):

Hydrogen Peroxide Quantification Kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 500 fluorometric tests (red fluorescence)

detection method

fluorometric

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

storage temp.

−20°C

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

Principle

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions. This kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates a red fluorescent product (λex = 540/λem = 590 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader.

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Rakesh Ruchel Khanikar et al.
RSC advances, 12(15), 9466-9472 (2022-04-16)
Cold atmospheric pressure (CAP) plasma has a profound effect on protein-protein interactions. In this work, we have highlighted the deactivation of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) spike protein by CAP plasma treatment. Complete deactivation of spike protein
Gaëtan Caravello et al.
Frontiers in immunology, 13, 858360-858360 (2022-05-03)
Microsporidia are obligate intracellular parasites able to infest specifically a large range of species, including insects. The knowledge about the biology of microsporidial infections remains confined to mostly descriptive studies, including molecular approaches such as transcriptomics or proteomics. Thus, functional
Eda Gjika et al.
ACS applied materials & interfaces, 10(11), 9269-9279 (2018-02-24)
Cold atmospheric plasma (CAP), an ionized gas operated at near-ambient temperatures, has been introduced as a new therapeutic opportunity for treating cancers. The effectiveness of the therapy has been linked to CAP-generated reactive oxygen and nitrogen species such as hydrogen
Yan Zhang et al.
Journal of nanobiotechnology, 19(1), 161-161 (2021-06-02)
As one typical cardiovascular disease, atherosclerosis severely endanger people' life and cause burden to people health and mentality. It has been extensively accepted that oxidative stress and inflammation closely correlate with the evolution of atherosclerotic plaques, and they directly participate
Wei Zhu et al.
Oxidative medicine and cellular longevity, 2019, 5305014-5305014 (2019-06-11)
Females develop kidney stones less frequently than males do. However, it is unclear if this gender difference is related to altered estrogen/estrogen receptor (ER) signaling. Here, we found that ER beta (ERβ) signals could suppress hepatic oxalate biosynthesis via transcriptional

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service