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MAK164

Sigma-Aldrich

Intracellular Hydrogen Peroxide Assay

sufficient for 200 fluorometric tests (green fluorescence)

Synonym(s):

Hydrogen Peroxide Detection in Cells

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About This Item

EC Number:
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 fluorometric tests (green fluorescence)

detection method

fluorometric

relevant disease(s)

aging/geriatric diseases; neurological disorders; pulmonary disorders; orthopedic diseases; cardiovascular diseases

storage temp.

−20°C

General description

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

Application

Intracellular hydrogen peroxide assay kit has been used to measure intracellular hydrogen peroxide levels.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

This kit is suitable for the detection of hydrogen peroxide in living cells and a variety of samples such as cell extracts and liquids.

Principle

The Intracellular Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in living cells and in a variety of other samples such as cellular extracts. This kit utilizes a unique cell-permeable sensor that generates a fluorescent product (λex = 490/λem = 520 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader, flow cytometer, or fluorescent microscope.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

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Qing You et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(17), 1903341-1903341 (2020-10-01)
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Nanna Cornelius et al.
Mitochondrion, 34, 103-114 (2017-03-07)
Spinocerebellar ataxia type 2 (SCA2) is a rare neurodegenerative disorder caused by a CAG repeat expansion in the ataxin-2 gene. We show increased oxidative stress, abnormalities in the antioxidant system, changes in complexes involved in oxidative phosphorylation and changes in
Carmen Sarcinelli et al.
Archaea (Vancouver, B.C.), 2016, 7424870-7424870 (2016-10-19)
Peroxiredoxins (Prxs) are ubiquitous thiol peroxidases that are involved in the reduction of peroxides. It has been reported that prokaryotic Prxs generally show greater structural robustness than their eukaryotic counterparts, making them less prone to inactivation by overoxidation. This difference
Dongdong Wang et al.
iScience, 9, 14-26 (2018-10-28)
Therapeutic effects of photodynamic therapy (PDT) remain largely limited because of tumor hypoxia. Herein, we report safe and versatile nanocatalysts (NCs) for endogenous oxygen generation and imaging-guided enhanced PDT. The NCs (named as PSP) are prepared by coating Prussian blue
Robert B Rosenblatt et al.
Theranostics, 11(2), 602-613 (2021-01-05)
Mechanical forces from non-ablative pulsed focused ultrasound (pFUS) generate pro-inflammatory tumor microenvironments (TME), marked by increased cytokines, chemokines, and trophic factors, as well as immune cell infiltration and reduced tumor growth. pFUS also causes DNA damage within tumors, which is

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