Skip to Content
Merck
All Photos(5)

Documents

G1N350

Sigma-Aldrich

GenElute Mammalian Genomic DNA Miniprep Kits

sufficient for 350 purifications

Synonym(s):

Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41105501
NACRES:
NA.55

usage

sufficient for 350 purifications

Quality Level

technique(s)

DNA purification: suitable

storage temp.

15-25°C

Looking for similar products? Visit Product Comparison Guide

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.

Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

GenElute Mammalian Genomic DNA Miniprep Kits has been used:
  • to extract DNA from fin samples
  • to extract the BSTUI restriction enzyme from the DNA
  • to isolate DNA from the blood samples extracted from caudal fin
  • to extract tissue and hair follicle DNA

The purified genomic DNA is ready for immediate use in downstream applications such as:
  • restriction endonuclease digestions
  • PCR
  • Southern blots
  • sequencing reactions
  • cloning
  • ligation

Features and Benefits

  • Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
  • Elution volume: 200 - 400 μl
  • Time required: 20 min after lysis
  • A260/A280 ratio: 1.6 - 1.9
  • Mechanical homogenization required: No

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • C2112Column Preparation SolutionSDS

  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biologySDS

  • R6148RNase A solutionSDS

recommended

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico
Parra-Bracamonte G M, et al.
Electronic journal of Biotechnology, 14(6), 11-11 (2011)
Gudrun Dittrich-Schröder et al.
Molecular ecology resources, 12(1), 109-115 (2011-09-29)
DNA extraction from minute hymenopterans and their larvae is difficult and challenging because of their small size indicating a low amount of starting material. Hence, 11 DNA extraction methods were compared to determine their efficacy in isolating DNA. Success of
Genetic shifts in the transition from wild to farmed white-tailed deer (Odocoileus virginianus) population
Hernandez-Mendoza P M, et al.
International Journal of Biodiversity Science, Ecosystem Services & Management, 10(1), 3-8 (2014)
Takahiro Seki et al.
Journal of controlled release : official journal of the Controlled Release Society, 156(3), 381-389 (2011-09-03)
Tumour Necrosis Factor alpha (TNF) is a pleiotropic pro-inflammatory cytokine with known vascular permeabilising activity. It is employed during isolated limb perfusion to enhance delivery of chemotherapeutic drugs into tumour tissue. The use of conditionally-replicating lytic viruses, so called 'oncolytic
Novel single nucleotide polymorphisms in candidate genes for growth in tilapia (Oreochromis niloticus)
Cuevas-Rodriguez B L, et al.
Revista Brasileira de Zootecnia, 45(6), 345-348 (2016)

Protocols

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.

The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.

See All

Related Content

GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service