NA2010

GenElute^™ Blood Genomic DNA Kit

sufficient for 70 purifications

• Synonym(s): Blood Genomic DNA, Gen Elute
• NACRES: NA.55
• UNSPSC Code: 41105501

Properties

• usage: sufficient for 70 purifications
• Quality Segment: 200
• technique(s): DNA purification: suitable
• input: blood
• test parameters: : <40 min kit run time, sample volume: 200 μL
• storage temp.: 15-25°C

Description

General description

Click here to view the kit protocol

The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Application

GenElute^™ Blood Genomic DNA Kit has been used:

• to extract DNA from samples of large granules (LG) and smaller granules (SG) obtained from vasectomized rabbits[1]
• to isolate DNA from the blood samples [2]
• to extract whole blood DNA from the left blood cells

The purified genomic DNA is ready for downstream applications such as:

• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning

Biochem/physiol Actions

The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.

The expected yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.

Features and Benefits

• Starting material: Up to 200 μl of fresh or aged blood
• Expected yield: Up to 10 μg
• Elution volume: 400 μl
• Time required: <40 min
• A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9
• Compatible with many anticoagulants, including EDTA, Heparin, and Sodium Citrate

• Typical yields 4-10 μg from 200 μL of whole blood
• Pure genomic DNA with a A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9
• Isolate DNA up to 50 kb in length

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• P2308

  Proteinase K from Tritirachium album, lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein 3

  SDS
• C2112

  Column Preparation Solution

  SDS
• R6148

  RNase A solution

  SDS

Safety Information

• pictograms: GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• target_organs: Respiratory system
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• hcodes: H302,H315,H318,H334,H335,H410,EUH430
• pcodes: P201 - P202 - P273 - P280 - P305 + P351 + P338 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3