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Urease from Canavalia ensiformis (Jack bean)

Name: Urease from <I>Canavalia ensiformis</I> (Jack bean)
Brand: SIGMA

powder, white, ~8 U/mg

Synonym(s):

Jack bean urease, Urea amidohydrolase

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About This Item

CAS Number:

9002-13-5

UNSPSC Code:

12352204

eCl@ss:

32160410

EC Number:

232-656-0

NACRES:

NA.54

MDL number:

MFCD00070858

EC Number:

3.5.1.5 (BRENDA, IUBMB)

Specific activity:

~8 U/mg

Biological source:

Canavalia ensiformis

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Properties

biological source

Canavalia ensiformis

Quality Segment

100

form

powder

specific activity

~8 U/mg

mol wt

M_r ~480000

color

white

storage temp.

−20°C

Description

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620

Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH. Urease is present in archaea, bacteria, unicellular eukaryotes and plants.

Application

Urease from Canavalia ensiformis may be used for urea determination of various samples, such as legumes . It may be useful for the detection of pathogens as well as heavy-metal ions.

Biochem/physiol Actions

Urease catalyzes the hydrolysis of urea into carbon dioxide and ammonia. Urease is involved in nitrogen metabolism and urea degradation. Urease from Canavalia ensiformis binds 2 nickel ions per subunit .

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol urea (Cat. No. 51459) per minute at pH 8.0 and 25°C

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This Item	U0251	U1500	U1875
Sigma-Aldrich 94281 Urease from Canavalia ensiformis (Jack bean)	Sigma-Aldrich U0251 Urease from Canavalia ensiformis (Jack bean)	Sigma-Aldrich U1500 Urease from Canavalia ensiformis (Jack bean)	Sigma-Aldrich U1875 Urease from Canavalia ensiformis (Jack bean)
description powder, white, ~8 U/mg	description Type C-3, powder, ≥600,000 units/g solid	description Type III, powder, 15,000-50,000 units/g solid	description Type III, glycerol solution, 500-800 units/mL
biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source Canavalia ensiformis	biological source Canavalia ensiformis
specific activity ~8 U/mg	specific activity ≥600,000 units/g solid	specific activity 15,000-50,000 units/g solid	specific activity -
form powder	form powder	form powder	form glycerol solution
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C
mol wt M_r ~480000	mol wt ~544620 Da	mol wt ~544620 Da	mol wt ~544620
color white	color -	color -	color -

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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