Sybr Green and Fluorescein sodium salt are both green dyes, and although their spectral ranges are close, it is important to consider their specific uses and characteristics. While Sybr Green is a popular dye for molecular use, Fluorescein sodium salt is a very economical product and may be useful for specific applications. However, there is no information comparing the two dyes for use in PCR.
46960
Fluorescein sodium salt
BioReagent, suitable for fluorescence
Synonym(s):
Acid Yellow 73, NaFl, Sodium fluorescein, Yellow No. 8, D&C;Yellow No. 8, NaFluo, Uranine
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About This Item
Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
Molecular Weight:
376.27
EC Number:
208-253-0
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3833041
Colour Index Number:
45350
Pricing and availability is not currently available.
product line
BioReagent
Quality Segment
form
powder
technique(s)
titration: suitable
fluorescence
λex 460 nm; λem 515 nm(lit.), λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
SMILES string
[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
InChI key
RGPLGPBQJOQFJS-UHFFFAOYSA-L
General description
Fluorescein Sodium Salt (FSS) is a xanthene dye having a polycrystalline structure with a monoclinic system. Fluorescein is a synthetic organic compound available as a dark orange/red powder soluble in water and alcohol.
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| technique(s) titration: suitable | technique(s) titration: suitable | technique(s) - | technique(s) - |
| suitability suitable for fluorescence | suitability - | suitability - | suitability - |
| fluorescence λex 460 nm; λem 515 nm(lit.), λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0 | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence - |
| form powder | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| product line BioReagent | product line - | product line - | product line - |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
423.7 °F - Pensky-Martens closed cup
flash_point_c
217.6 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves, type N95 (US)
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Joshua L Turnbull et al.
Journal of the American Chemical Society, 143(16), 6194-6201 (2021-04-03)
Xanthene fluorophores, like fluorescein, have been versatile molecules across diverse fields of chemistry and life sciences. Despite the ubiquity of 3-carboxy and 3-sulfonofluorescein for the last 150 years, to date, no reports of 3-phosphonofluorescein exist. Here, we report the synthesis
Tomoaki Murakami et al.
Ophthalmology, 120(12), 2596-2603 (2013-08-21)
To study the association between the fluorescence levels on fluorescein angiography images and the characteristics on spectral-domain optical coherence tomography (SD OCT) images in diabetic macular edema (DME). Retrospective, observational, cross-sectional study. One hundred sixty-seven consecutive eyes of 116 patients
Christopher Price et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 26(2), 277-285 (2010-08-18)
Since proposed by Piekarski and Munro in 1977, load-induced fluid flow through the bone lacunar-canalicular system (LCS) has been accepted as critical for bone metabolism, mechanotransduction, and adaptation. However, direct unequivocal observation and quantification of load-induced fluid and solute convection
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PH004931-1MG | 04061822881242 |
| 46960-500G-F | 04061832359021 |
| 46960-100G-F | 04061832359014 |
| 46960-25G-F | 04061822703773 |
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For Fluorescein sodium salt 46960, is there a fluorescence value for a positive PCR sample, and how would this value translate as a concentration of this powder?
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