For product F6377 the dye is tested in water at a low concentration. Conn's Biological Stains, 10th Edition states the sodium salt in soluble in water up to 50%. It is expected the dye should dissolve in HBSS but there is no supportive data. There is also no solution stability information available. Although the dry powder should be stored at room temperature, solutions are probably best kept at 2-8°C.
F6377
Fluorescein sodium salt
Powder
Fluorescein sodium salt
Synonym(s):
Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine
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About This Item
Empirical Formula (Hill Notation):
C20H10Na2O5
CAS Number:
Molecular Weight:
376.27
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
208-253-0
MDL number:
Beilstein/REAXYS Number:
3833041
Colour Index Number:
45350
Pricing and availability is not currently available.
Product Name
Fluorescein sodium salt, used as fluorescent tracer
Quality Segment
form
powder
technique(s)
titration: suitable
pH
8.3 (20 °C, 10 g/L)
mp
>300 °C
solubility
H2O: 1 mg/mL
density
0.78 g/cm3 at 29.6 °C (85.3 °F at 699.7 hPa)
fluorescence
λex 460 nm; λem 515 nm(lit.)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
InChI
1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
InChI key
RGPLGPBQJOQFJS-UHFFFAOYSA-L
General description
Fluorescein is a xanthene dye and is a common labeling agent in biology.
Application
Fluorescein sodium salt has been used as a solute to study microvessel permeability. It has been used as a test molecule to study blood barrier penetration.
Water soluble fluorescent dye used in permeability and vascular perfusion studies due to its small molecular radius.[1] Sodium fluorescein is used as a fluorescent tracer to study blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) permeability in rodent models.[2][3][4] The dye was used as a probe substrate in the study of organic anion transporting polypeptide (OATP) mediated drug transport in hepatocytes.[5] Sodium fluorescein was also used to monitor in situ solute transportation into bone through fluorescence recovery after photobleaching (FRAP).[6]
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This Item | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) - | technique(s) - |
| fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence λex 460 nm; λem 515 nm(lit.) | fluorescence - |
| form powder | form - | form powder | form powder |
| solubility H2O: 1 mg/mL | solubility - | solubility - | solubility 500 g/L |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) - | application(s) pharma/biopharma processes |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
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Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
423.7 °F - Pensky-Martens closed cup
flash_point_c
217.6 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves, type N95 (US)
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Can I make a stock solution of this in HBSS? If yes, what is the stability and recommended storage conditions?
1 answer-
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Hello, I would like to ask if this product's fluorescence can be quenched by paraformaldehyde (PFA)? Thank you.
1 answer-
The preferred fixative for various staining procedures is often freshly prepared paraformaldehyde solution. Paraformaldehyde solution is not known to quench fluorescein sodium salt or other fluorescent molecules. However, no specific analysis been performed as part of the quality control testing performed on product F6377 to ensure fluorescence is not quenched.
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What is the purity of Fluorescein sodium salt (CAS number 518-47-8, article number F6377-100G)?
1 answer-
This purity of this product is not determined.
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Can this product be autoclaved once dissolved? How long can a concentrated dissolved stock solution be stored for?
1 answer-
The stability of Fluorescein under autoclave conditions has not been determined. Solutions have typically been filter sterilized. Long term solution stability has also not been evaluated. However, solutions of Fluorescein Diacetate are stable at -20°C in ethanol when protected from light. Similar stability would be expected with the sodium salt form.
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Hello, I am interested in Fluorescein or Fluoresein sodium salt to perform angiography in the living mouse and would like to know if your product has a pharmacological grade. Which product is used for angiography?
1 answer-
Unfortunately, a pharmacological grade Fluorescein or Fluorescein sodium salt is not available at this time.
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Is F6377 soluble in ethanol?
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The substance is soluble in water (500 g/l) at 20°C and slightly soluble in ethanol, but insoluble in benzene, chloroform, and ether.
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