The preparation instructions and protocol for this kit are published in the Certificate of Analysis. Please see the link below to access a sample of lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/rab0436#product-documentation
RAB0436
Human Osteopontin ELISA Kit
for serum, plasma, cell culture supernatant and urine
Synonym(s):
OPN
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.32
Pricing and availability is not currently available.
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species reactivity
human
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type serum
sample type plasma
sample type urine
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 50 pg/mL
standard curve range: 74.07-18000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... SPP1(6696)
General description
The Human OPN ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human OPN in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Human OPN
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Osteopontin (OPN) is a secreted glycoprotein and is assumed to be associated with various cellular processes such as adhesion, migration, and biomineralization in diverse tissues. Osteopontin acts as a ligand to αvβ3 integrin and possibly other receptors.[3] In addition to its involvement in mineralized matrix formation, osteopontin can also function as a cytokine that stimulates the release of IFNγ (interferon γ) and IL-12 (interleukin 12), while inhibiting the production of IL-10. Osteopontin is involved in inflammation and also regulates immunity against infectious diseases. It also co-stimulates T cell proliferation.[4] Osteopontin has proinflammatory functions and is associated with multiple sclerosis.[5] It participates in inflammation in Crohn′s Disease.[6] Osteopontin plays a crucial role in bone remodelling and retards ectopic calcification by inhibiting the formation of hydroxyapatite. OPN is thought to prevent renal stone formation by suppressing nucleation, growth and aggregation of calcium oxalate crystals.[7]
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
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Can Osteopontin Be Considered a Biomarker for Endometriosis?
Albu D R, et al.
Revista de Chimie (Bucharest, Romania), 13(3), 1-1 (2017)
EFFECT OF MITRAL VALVE SURGERY FOR MITRAL REGURGITATION ON SERUM OSTEOPONTIN LEVELS
Seddik M, et al.
Al-Azhar Assiut Medical Journal, 13(3), 1-1 (2015)
Octavio Gamaliel Aztatzi-Aguilar et al.
PloS one, 16(3), e0249057-e0249057 (2021-03-26)
Osteopontin (OPN) is a protein involved in inflammatory illnesses such as fibrosis and cancer; its overexpression in cardiovascular diseases promotes the biomineralization of blood vessels and other soft tissues. Moreover, there is an active component of oxidative stress related with
The influence of the proinflammatory cytokine, osteopontin, on autoimmune demyelinating disease.
Chabas D, et al.
Science, 294, 1731-1735 (2001)
Post-translational modification and proteolytic processing of urinary osteopontin.
Christensen B, et al.
The Biochemical Journal, 411, 53-61 (2008)
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How do I reconstitute the lyophilized human osteopontin protein?
1 answer-
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As I was looking through the general sandwich assay protocol, I was curious about what the wash buffer is diluted in. Is there no serial dilution step for that standard as the kit contains a 1x and 5x osteopontin standard? What's the kit's protocol?
1 answer-
The protocol can be found in the Certificate of Analysis in the document section of the product detail page. For the RAB series ELISA, the COA can apply to any lot as the standard concentration, reagent preparation and assay procedures are the same for each lot.
A copy of the protocol can be found at the link below: https://www.sigmaaldrich.com/coa/SIGMA/RAB0436/SAMPLE
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