This protein has the sequence of Mouse myeloma cell line, NS0-derived human Osteopontin/OPN protein, Ile17-Asn300, with a C-terminal 6-His tag. The amino acids of Ile17-Asn300 can be found in this article; https://www.ncbi.nlm.nih.gov/protein/NP_000573.1
O4264
Human Osteopontin
recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
Synonym(s):
Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)
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Product Name
Osteopontin human, recombinant, expressed in NSO cells, ≥95% (SDS-PAGE), lyophilized powder, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in NSO cells
sterility
sterile
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
60-65 kDa by SDS-PAGE (reducing)
packaging
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
shipped in
ambient
storage temp.
−20°C
Gene Information
human ... SPP1(6696)
General description
Application
Anti-Osteopontin antibody produced in rabbit has been used for ELISA.[4]
Biochem/physiol Actions
A secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig, and bovine OPN share 40% amino acid sequence identity. Secreted phosphoprotein 1 (SPP1) or osteopontin modulates inflammatory responses and glucose metabolism. It is a chemotactic factor and has a role in immune cell recruitment. The protein is also associated with cell growth, migration and differentiation. SPP1 attaches osteoclasts to the bone matrix. It is also involved in atherosclerotic process and bone mineralization.[5][6] It is a marker of many cancers.[7][1][2]
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
Analysis Note
Bioactivity Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. The ED50 for this effect is is 0.100-0.600 ug/mL.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Osteopontin Fragments with Intact Thrombin-Sensitive Site Circulate in Cervical Cancer Patients
Danny TM
PLoS ONE (2016)
Expanded comparative mapping between man
and rabbit and detection of a new conserved
segment between HSA22 and OCU4
and rabbit and detection of a new conserved
segment between HSA22 and OCU4
C Chantry
Cytogenetic and genome research (2005)
Polycystic ovary syndrome is associated with increased osteopontin levels
European Journal of Endocrinology (2015)
Osteopontin That Is Elevated in the Airways during COPD Impairs the Antibacterial Activity of Common Innate Antibiotics
Anele Gela
PLoS ONE (2016)
Utility of Plasma Osteopontin Levels in Management of Community-Acquired Pneumonia
Jer-Hwa Chang
International Journal of Medical Sciences (2016)
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What is the amino acid sequence of Osteopontin human O4264?
What is the amino acid sequence of Osteopontin human O4264?
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