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77140

Sigma-Aldrich

Peptidoglycan from Staphylococcus aureus

cell wall component

Synonym(s):

Peptidoglycan from S. aureus

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About This Item

MDL number:
MFCD00212486
UNSPSC Code:
12352201
NACRES:
NA.32
Pricing and availability is not currently available.

Quality Level

100

form

lyophilized powder

solubility

soluble, hazy to turbid, colorless to faintly yellow

storage temp.

−20°C

Related Categories

Application

Peptidoglycan from Staphylococcus aureus has been used:
  • for the stimulation of lymphocytes: peptidoglycan activates the toll-like receptor 2 (TLR2), present in mammalian cells. It works as an antagonist of Poly (I:C)[1]
  • for g-type lysozyme stimulation by intraperitoneally inoculating it into sole specimens[2]
  • in phosphate buffer saline and injected into Labeo rohita fingerlings, to study its effect on nucleotide-binding oligomerization domain-2 (NOD-2) gene expression[3]

Biochem/physiol Actions

The cell wall of gram positive bacteria is largely made up of peptidoglycan.[4] It is composed of polysaccharide chains interlinked by peptide bridges. This peptidoglycan gives a certain rigidity to the cell wall and gives the cell mechanical strength.[5] It is a surface molecule which is known to stimulate immune reactions including cytokine production to fever.[6]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Major component of the cell wall in gram positive organisms. Consists of a glycan backbone with alternating β 1-4 linked residues of N-acetyl-D-glucosamine and muramic acid. The immunological activities of bacterial peptidoglycans[7]; Peptidoglycan types of bacterial cell walls and their taxonomic implications[8]; Antibody response to peptidoglycan during staphylococcal infections[9]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCJ7175
BCCF2500
BCCC1420
BCBZ7206
BCBW7713

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ABC transporters involved in the biogenesis of the outer membrane in gram-negative bacteria
Narita S
Bioscience, Biotechnology, and Biochemistry, 75(6), 1044-1054 (2011)
Sara Abouelasrar Salama et al.
Mediators of inflammation, 2020, 6087109-6087109 (2020-07-23)
The serum amyloid A (SAA) gene family is highly conserved and encodes acute phase proteins that are upregulated in response to inflammatory triggers. Over the years, a considerable amount of literature has been published attributing a wide range of biological
Molecular characterization of nucleotide binding and oligomerization domain (NOD)-2, analysis of its inductive expression and down-stream signaling following ligands exposure and bacterial infection in rohu (Labeo rohita)
Swain B, et al.
Developmental and Comparative Immunology, 36(1), 93-103 (2012)
Peptidoglycan types of bacterial cell walls and their taxonomic implications.
K H Schleifer et al.
Bacteriological reviews, 36(4), 407-477 (1972-12-01)
Xue Kong et al.
Frontiers in physiology, 9, 1476-1476 (2018-11-09)
Vesicomyidae clams are common species living in cold seeps, which incorporates symbiotic bacteria into their body maintaining endosymbiosis relationship. As members of pattern recognition receptor (PRR) family, peptidoglycan recognition proteins (PGRPs) recognize pathogen associated molecular patterns and play an important
View All Related Papers

Articles

Peptidoglycan Structure and Biosynthesis

Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.

Peptidoglycan Structure and Biosynthesis

Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.

Peptidoglycan Structure and Biosynthesis

Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.

Peptidoglycan Structure and Biosynthesis

Peptidoglycan structure: alternating GlcNAc and N-acetylmuramic acid cross-linked to peptides form the basic backbone.

Questions

1–5 of 5 Questions  

  1. When I dissolve the 10mg PGN in 10mL of ultrapure water, there are white chunks that float to the bottom. Are there any recommendations for making these particles more uniform in the suspension? Or a better way to dissolve the PGN?

    1 answer

    1. This product is not tested for solubility. Peptidoglycans are only minimally soluble and will often produce a hazy or turbid solution. Sonication and/or further dilution may improve the solubility.

      The product issue you are reporting should be investigated by our Technical Service team, who can assist you with further troubleshooting and/or investigation. We kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice and click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

  2. Does Product 77140, Peptidoglycan, have a recommended retest date?

    1 answer

    1. If a product has a recommended retest date this would be on the Certificate of Analysis found at our website. If no recommended retest date is found on the Certificate of Analysis, then the guarantee of quality is one year from the time of delivery for unopened bottles stored at the recommended temperature.

      Helpful?

  3. How can solutions of Product 77140, Peptidoglycan, be stored?

    1 answer

    1. Sigma has not evaluated the solution stability of solutions.  It is therefore recommended that fresh solutions be prepared.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Can Product 77140, Peptidoglycan, be solubilized in water?

    1 answer

    1. No, the product is not soluble in water, or in other solvents for that matter. It forms a suspension in water.

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