R4777
Anti-Ran antibody,Mouse monoclonal
clone ARAN1, purified from hybridoma cell culture
Synonym(s):
Anti-ARA24, Anti-Gsp1, Anti-TC4
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
ARAN1, monoclonal
form
buffered aqueous solution
species reactivity
human, Xenopus, bovine, mouse, hamster
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total extract of Jurkat cells
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... RAN(5901)
mouse ... Ran(19384)
Related Categories
General description
Monoclonal Anti-Ran (mouse IgG2b isotype) is derived from the ARAN1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BDF1 mice immunized with denatured recombinant human Ran. Ran is a small guanine trinucleotide phosphatase (GTPase) of the Ras superfamily that like other GTPases exist in two states: bound to guanine trinucleotide phosphate (GTP) or to guanine dinucleotide phosphate (GDP). The bound form is localized to the nucleus.
Immunogen
denatured recombinant human Ran.
Application
Monoclonal Anti-Ran antibody produced in mouse has been used in:
- western blotting
- immunoprecipitation
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
Biochem/physiol Actions
Ran plays an important function in the directionality of nuclear transport. In this form it is important for the termination of the import reaction which is mediated by a complex of the cargo protein with importin-β and its adapter protein importin-α. The binding of RanGTP to importin-β causes the release of the cargo protein from the importin-α/importin-β complex. The import receptor (importin-β or transportin) is then exported out of the nucleus as a RanGTP complex and is released into the cytoplasm when RanGTP is converted to RanGDP by Ran-GAP1 (GTPase-activating protein). Export receptors respond in the opposite way by binding cargo with much higher affinity in the presence of RanGTP and exporting them out of the nucleus as RanGTP complexes. The exported cargo is released when Ran GAP1 converts RanGTP to RanGDP. In order to maintain the import/export cycle, Ran has to return to the nucleus. This is achieved by a small protein called nuclear transport factor 2 (NTF2) also known as p10, that binds RanGDP in the cytoplasm, and delivers it back into the nucleus. In the nucleus RanGDP encounters RanGEF (guanine nucleotide exchange factor) and undergoes nucleotide exchange to become RanGTP.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins
Rexach M and Blobel G
Cell, 83(5), 683-692 (1995)
Masahiro Nagai et al.
FEBS letters, 585(5), 791-796 (2011-02-12)
The small GTPase Ran plays important roles in multiple aspects of cellular function. Maximal RanGAP activity is achieved with the aid of RanBP1 and/or presumably of RanBP2. Here, we show that RanBP1-knockout mice are unexpectedly viable, and exhibit male infertility
A mass spectrometry-based approach for the identification of Kpnβ1 binding partners in cancer cells.
Michael O Okpara et al.
Scientific reports, 12(1), 20171-20171 (2022-11-24)
Karyopherin beta 1 (Kpnβ1) is the principal nuclear importer of cargo proteins and plays a role in many cellular processes. Its expression is upregulated in cancer and essential for cancer cell viability, thus the identification of its binding partners might
RNASEK is a V-ATPase-associated factor required for endocytosis and the replication of rhinovirus, influenza A virus, and dengue virus
Perreira JM, et al.
Cell Reports, 12(5), 850-863 (2015)
The role of Ran in nuclear function
Azuma Y and Dasso M
Current Opinion in Cell Biology, 12(3), 302-307 (2000)
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