D2693
Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution, sufficient for 10 blots
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About This Item
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biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
usage
sufficient for 10 blots
mol wt
antigen 32 kDa
species reactivity
mouse, human
technique(s)
dot blot: 1:1,000
western blot: 1:1,000
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PPP1R1B(84152)
mouse ... Ppp1r1b(19049)
General description
DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine.
Immunogen
synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75.
Application
Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates.
Biochem/physiol Actions
Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac®.
Target description
DARPP32 phosphorylated on Thr75 acts as an inhibitor of PKA.
Physical form
Solution at 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.
Other Notes
The labeling by the antibody to DARPP32 Thr75 is markedly increased by okadaic acid treatment.
Legal Information
Prozac is a registered trademark of Eli Lilly and Co.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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R E Maldve et al.
Nature neuroscience, 5(7), 641-648 (2002-06-18)
The medium spiny neurons of the nucleus accumbens receive both an excitatory glutamatergic input from forebrain and a dopaminergic input from the ventral tegmental area. This integration point may constitute a locus whereby the N-methyl-D-aspartate (NMDA)-subtype of glutamate receptors promotes
Maria Lindskog et al.
Nature, 418(6899), 774-778 (2002-08-16)
Caffeine has been imbibed since ancient times in tea and coffee, and more recently in colas. Caffeine owes its psychostimulant action to a blockade of adenosine A(2A) receptors, but little is known about its intracellular mechanism of action. Here we
J A Bibb et al.
Nature, 402(6762), 669-671 (1999-12-22)
The physiological state of the cell is controlled by signal transduction mechanisms which regulate the balance between protein kinase and protein phosphatase activities. Here we report that a single protein can, depending on which particular amino-acid residue is phosphorylated, function
Per Svenningsson et al.
Science (New York, N.Y.), 302(5649), 1412-1415 (2003-11-25)
Three distinct classes of drugs: dopaminergic agonists (such as D-amphetamine), serotonergic agonists (such as LSD), and glutamatergic antagonists (such as PCP) all induce psychotomimetic states in experimental animals that closely resemble schizophrenia symptoms in humans. Here we implicate a common
A A Fienberg et al.
Science (New York, N.Y.), 281(5378), 838-842 (1998-08-07)
Dopaminergic neurons exert a major modulatory effect on the forebrain. Dopamine and adenosine 3',5'-monophosphate-regulated phosphoprotein (32 kilodaltons) (DARPP-32), which is enriched in all neurons that receive a dopaminergic input, is converted in response to dopamine into a potent protein phosphatase
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