D1556

OptiPrep^™ Density Gradient Medium

used for cell and subcellular organelle isolation

• Synonym(s): Iodixanol solution
• CAS Number: 92339-11-2
• MDL number: MFCD00867965
• UNSPSC Code: 12352207
• PubChem Substance ID: 329798703
• NACRES: NA.75

Properties

• sterility: sterile
• form: liquid
• density: 1.319-1.321 g/mL
• application(s): hematology; histology
• SMILES string: CC(=O)N(CC(O)CN(C(C)=O)c1c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c1I)c2c(I)c(C(=O)NCC(O)CO)c(I)c(C(=O)NCC(O)CO)c2I
• InChI: 1S/C35H44I6N6O15/c1-13(52)46(30-26(38)20(32(59)42-3-15(54)9-48)24(36)21(27(30)39)33(60)43-4-16(55)10-49)7-19(58)8-47(14(2)53)31-28(40)22(34(61)44-5-17(56)11-50)25(37)23(29(31)41)35(62)45-6-18(57)12-51/h15-19,48-51,54-58H,3-12H2,1-2H3,(H,42,59)(H,43,60)(H,44,61)(H,45,62)
• InChI key: NBQNWMBBSKPBAY-UHFFFAOYSA-N

Description

General description

OptiPrep^™ Density Gradient Medium, also known as iodixanol, is a low osmolarity, non-ionic iodinated density gradient medium. It is a sterile and endotoxin-tested solution that comprises 60% (w/v) iodixanol in water, designed for the in vitro isolation of biological particles. It may be used for fractionation and separation of cell and subcellular organelles.

Application

OptiPrep^™ has been used to isolate molecules, cells, and subcellular organelles, including DNA, cytokines, lysosomes, endoplasmic reticulum, peroxisomes, T lymphocytes, and natural killer cells. It has also been used for the following applications:

• to investigate the efficiency of single-cell encapsulation.
• the gradient-based isolation of senescent cancer cells.
• isolation of non-enveloped virion isolation from bronchoalveolar lavage.
• isolation of lipoproteins and macromolecules
• purification of virions
• purification of neuron cells

Features and Benefits

OptiPrep^™ has numerous biocompatible properties that make it ideal for use.

• It is biologically inert and does not interfere with assays for marker enzymes.
• It is non-ionic, non-toxic, and has low osmotic pressure.
• It forms spontaneous gradients that are stabler and easier to prepare than sucrose.

Other Notes

60% (w/v) solution of iodixanol in water (sterile).

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable