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79972

Sigma-Aldrich

7-(Isobutoxycarbonyloxy)-3H-phenoxazin-3-one

suitable for fluorescence, ≥97.0% (HPLC)

Synonym(s):

O7-(Isobutyloxycarbonyl)resorufin

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About This Item

Empirical Formula (Hill Notation):
C17H15NO5
CAS Number:
251292-24-7
Molecular Weight:
313.30
MDL number:
MFCD03791103
UNSPSC Code:
12352200
PubChem Substance ID:
57652821
NACRES:
NA.32

Assay

≥97.0% (HPLC)

form

powder

fluorescence

λex 500 nm; λem ~593 nm in 0.1 M Tris pH 8.0 (after cleavage by esterase)

suitability

suitable for fluorescence

SMILES string

CC(C)COC(=O)Oc1ccc2N=C3C=CC(=O)C=C3Oc2c1

InChI

1S/C17H15NO5/c1-10(2)9-21-17(20)22-12-4-6-14-16(8-12)23-15-7-11(19)3-5-13(15)18-14/h3-8,10H,9H2,1-2H3

InChI key

RVDFSVVDUVHHKE-UHFFFAOYSA-N

Other Notes

Fluorogenic indicator for cell viability, cell permeant, ~2-times higher sensitivity than calcein-AM; additionally, 80-90 nm more long-wave shift of spectra. Shows esterase activity

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
429365/1

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M. Ishiyama et al.
Analytical Sciences, 15, 1025-1025 (1999)

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