ZRB1546
Anti-Phospho-AKT1-(Ser473) Antibody, clone 1O14 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
RAC-alpha serine/threonine-protein kinase;EC:2.7.11.1;Protein kinase B;PKB;Protein kinase B alpha;PKB alpha;Proto-oncogene c-Akt;RAC-PK-alpha;Akt alpha
About This Item
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1O14, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 55.69 kDa
observed mol wt ~66 kDa
purified by
using Protein A
species reactivity
human, mouse
species reactivity (predicted by homology)
feline, rat, bovine, monkey, canine, zebrafish, porcine
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype
IgG
epitope sequence
C-terminus
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer473/pSer473)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from NIH3T3 cells treated with platelet derived growth factor (PDGF).
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected AKT1 in lysate from NIH3T3 cells serum starved overnight and treated with PDGF (100 ng/mL; 20 min).
Tested applications
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected AKT1 in lysate from HEK293 cells serum starved overnight and treated with Insulin (500 nM; 10 min).
Peptide Inhibition Assay: Target band detection in a lysate from HEK293 cells treated with insulin was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Affinity Binding Assay: A representative lot of this antibody bound AKT1 peptide with a KD of 1.1 x 10-8 in an affinity binding assay.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Phospho-AKT1-(Ser473) in human breast cancer and human melanoma tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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