T8328

Anti-β-Tubulin antibody, Mouse monoclonal

~2.0 mg/mL, clone AA2, purified from hybridoma cell culture

• Synonym(s): Beta Tubulin Antibody Sigma, Beta Tubulin Antibody Sigma - Monoclonal Anti-β-Tubulin antibody produced in mouse, Anti-TUBB, Anti-Tubulin, beta
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00145892
• Conjugate: unconjugated
• Clone: AA2, monoclonal
• Application: ICC, IHC, IP, WB
• Citations: 371

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: AA2, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~50 kDa
• species reactivity: human, mouse, rat, bovine
• packaging: antibody small pack of 25 μL
• concentration: ~2.0 mg/mL
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using Neuro-2a cell extract
• isotype: IgG1
• UniProt accession no.: P07437
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBB(203068)

Description

General description

β-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V) and βVI (class VI)).

Tubulin βIII (TUBB3) is a constituent of microtubules. It is expressed in mature neurons. It is located on human chromosome 16q24.

Tubulin, a 100kDa protein is present in all cells as a heterodimer of two similar polypeptides α and β, which assemble to form microtubules. Monoclonal anti-β-tubulin antibody can be used to visualize HRP signal using enhanced chemiluminescence.

Immunogen

Purified bovine tubulin

Application

Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting and immunofluorescence staining.

Anti-Tubulin βIII antibody has been used in western blot analysis.

Monoclonal anti-β-tubulin antibody (diluted 1: 5000) can be used as a primary antibody in immunofluorescence staining of mammalian oocytes. It can also be used in numerous immunochemical techniques like-, immunocytochemistry, immunoblotting (50kD approx), immunohistochemistry and immunoprecipitation.

Biochem/physiol Actions

β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
B2702 peptide binds to β-tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations.

Mouse anti-β-tubulin antibody reacts specifically with β tubulin, types I, II, III, and IV of bovine, rat, mouse and human.

Proper organization of microtubules mediated by tubulin is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions.

Upregulation of tubulin βIII (TUBB3) shows vigorous tumor characteristics and genetic instability in urinary bladder cancer. It participates in the separation of chromosome. Individuals with congenital fibrosis of the extraocular muscle type 3 (CFEOM3) show mutations in TUBB3. βIII-tubulin modulates glucose metabolism, thereby transforms glucose starvation stress signaling to develop the multiplication of cell and survival in non-small cell lung cancer cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)